PMID- 18344617
OWN - NLM
STAT- MEDLINE
DCOM- 20080725
LR  - 20190513
IS  - 0449-3060 (Print)
IS  - 0449-3060 (Linking)
VI  - 49
IP  - 3
DP  - 2008 May
TI  - Differential induction from X-irradiated human peripheral blood monocytes to 
      dendritic cells.
PG  - 293-303
AB  - Dendritic cells (DCs) are a type of antigen-presenting cell which plays an 
      essential role in the immune system. To clarify the influences of ionizing 
      radiation on the differentiation to DCs, we focused on human peripheral blood 
      monocytes and investigated whether X-irradiated monocytes can differentiate into 
      DCs. The non-irradiated monocytes and 5 Gy-irradiated monocytes were induced into 
      immature DCs (iDCs) and mature DCs (mDCs) with appropriate cytokine stimulation, 
      and the induced cells from each monocyte expressed each DC-expressing surface 
      antigen such as CD40, CD86 and HLA-DR. However, the expression levels of CD40 and 
      CD86 on the iDCs derived from the 5 Gy-irradiated monocytes were higher than 
      those of iDCs derived from non-irradiated monocytes. Furthermore, the mDCs 
      derived from 5 Gy-irradiated monocytes had significantly less ability to 
      stimulate allogeneic T cells in comparison to the mDCs derived from 
      non-irradiated monocytes. There were no significant differences in the 
      phagocytotic activity of the iDCs and cytokines detected in the supernatants 
      conditioned by the DCs from the non-irradiated and irradiated monocytes. These 
      results suggest that human monocytes which are exposed to ionizing radiation can 
      thus differentiate into DCs, but there is a tendency that X-irradiation leads to 
      an impairment of the function of DCs.
FAU - Yoshino, Hironori
AU  - Yoshino H
AD  - Department of Radiological Life Sciences, Hirosaki University Graduate School of 
      Health Sciences, Hirosaki, Aomori, Japan.
FAU - Takahashi, Kenji
AU  - Takahashi K
FAU - Monzen, Satoru
AU  - Monzen S
FAU - Kashiwakura, Ikuo
AU  - Kashiwakura I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080314
PL  - England
TA  - J Radiat Res
JT  - Journal of radiation research
JID - 0376611
SB  - IM
MH  - Cell Differentiation/*radiation effects
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Monocytes/*physiology/*radiation effects
MH  - Phagocytosis
EDAT- 2008/03/18 09:00
MHDA- 2008/07/26 09:00
CRDT- 2008/03/18 09:00
PHST- 2008/03/18 09:00 [pubmed]
PHST- 2008/07/26 09:00 [medline]
PHST- 2008/03/18 09:00 [entrez]
AID - JST.JSTAGE/jrr/07122 [pii]
AID - 10.1269/jrr.07122 [doi]
PST - ppublish
SO  - J Radiat Res. 2008 May;49(3):293-303. doi: 10.1269/jrr.07122. Epub 2008 Mar 14.