PMID- 1834617
OWN - NLM
STAT- MEDLINE
DCOM- 19911211
LR  - 20190903
IS  - 0018-2214 (Print)
IS  - 0018-2214 (Linking)
VI  - 23
IP  - 6
DP  - 1991 Jun
TI  - Histochemical profiles of motoneurons innervating muscle fibres with different 
      activity patterns in the zebrafish, Brachydanio rerio.
PG  - 273-80
AB  - Enzyme histochemical profiles of spinal motoneurons in the zebrafish were 
      determined. Five enzymes of glucose metabolism were chosen: glucose-6-phosphate 
      dehydrogenase (G6PDH), hexokinase (HK), phosphofructokinase (PFK), succinate 
      dehydrogenase (SDH) and NADH tetrazolium reductase (NADH-TR). Motoneurons were 
      traced with Fluorogold and classified as those that innervate white muscle fibres 
      (W-MNs) and those that innervate red and intermediate muscle fibres (R/I-MNs). 
      The average enzyme activities per volume of tissue in the somata of both 
      populations differed at most by 25%. Both the average soma volume and the average 
      number of muscle fibres innervated are three times larger for the W-MNs than for 
      the R/I-MNs. This suggests that the total amount of enzyme activity within a 
      neuron soma matches target size. In the R/I-MNs, the activities of SDH and 
      NADH-TR were closely correlated (correlation coefficient, r = 0.99; p less than 
      0.05) and HK activity correlated well with G6PDH activity (r = 0.94; p less than 
      0.05), but not with PFK (r = 0.64; p greater than 0.05). In the W-MNs, there was 
      no correlation between SDH and NADH-TR (r = -0.59; p greater than 0.05) or 
      between HK and G6PDH (r = 0.50; p greater than 0.05) and the correlation 
      coefficient between HK and PFK activity was close to zero (r = 0.04; p greater 
      than 0.05). It was concluded that in the R/I-MNs, which are continuously active, 
      firing activity is fuelled by oxidative metabolism. We suggest that in the W-MNs 
      glucose is stored in the form of glycogen and that, despite high levels of 
      NADH-TR present, the energy for intermittent firing activity is provided by 
      glycolysis.
FAU - De Graaf, F
AU  - De Graaf F
AD  - Department of Experimental Zoology, University of Amsterdam, The Netherlands.
FAU - Van Raamsdonk, W
AU  - Van Raamsdonk W
FAU - Van Asselt, E
AU  - Van Asselt E
FAU - Diegenbach, P C
AU  - Diegenbach PC
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Histochem J
JT  - The Histochemical journal
JID - 0163161
RN  - 0 (Tetrazolium Salts)
RN  - 0468TBH014 (4-anisyltetrazolium blue)
RN  - 0U46U6E8UK (NAD)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 1.3.99.1 (Succinate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
SB  - IM
MH  - Animals
MH  - Cell Compartmentation
MH  - Glucosephosphate Dehydrogenase/analysis
MH  - Glycolysis
MH  - Hexokinase/analysis
MH  - Motor Neurons/enzymology/*metabolism
MH  - Muscles/*innervation
MH  - NAD/analysis
MH  - Oxidation-Reduction
MH  - Phosphofructokinase-1/analysis
MH  - Spinal Cord/anatomy & histology/metabolism
MH  - Succinate Dehydrogenase/analysis
MH  - Tetrazolium Salts
MH  - Zebrafish
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 10.1007/BF01045046 [doi]
PST - ppublish
SO  - Histochem J. 1991 Jun;23(6):273-80. doi: 10.1007/BF01045046.