PMID- 18346946
OWN - NLM
STAT- MEDLINE
DCOM- 20080808
LR  - 20161124
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 865
IP  - 1-2
DP  - 2008 Apr 1
TI  - Simultaneous determination of calycosin-7-O-beta-D-glucoside, ononin, 
      astragaloside IV, astragaloside I and ferulic acid in rat plasma after oral 
      administration of Danggui Buxue Tang extract for their pharmacokinetic studies by 
      liquid chromatography-mass spectrometry.
PG  - 99-105
LID - 10.1016/j.jchromb.2008.02.024 [doi]
AB  - A sensitive and reliable high-performance liquid chromatography-mass spectrometry 
      (HPLC-MS) was developed and validated for simultaneous quantification of five 
      main bioactive components, i.e., calycosin-7-O-beta-D-glucoside, ononin, 
      astragaloside IV, astragaloside I and ferulic acid in rat plasma after oral 
      administration of Danggui Buxue Tang (DBT) extract. Plasma samples were extracted 
      with solid-phase extraction (SPE) separated on an Inertsil ZORBAX C(18) column 
      and detected by MS with electrospray ionization (ESI) interface in negative 
      selective ion monitoring (SIM) mode. Calibration curves offered linear ranges of 
      two orders of magnitude with r(2)>0.99. The method had the lower limit 
      quantification of 0.55, 0.46, 1.07, 1.12 and 4.6 ng/mL for 
      calycosin-7-O-beta-D-glucoside, ononin, astragaloside IV, astragaloside I and 
      ferulic acid, respectively, with precision less than 10%. The RSD of intra- and 
      inter-day variations ranged from 2.10% to 6.19% and 2.37% to 6.72%. This 
      developed method was subsequently applied to pharmacokinetic studies of the five 
      compounds in rats successfully.
FAU - Wen, Xiao-Dong
AU  - Wen XD
AD  - Key Laboratory of Modern Chinese Medicines, Ministry of Education, Department of 
      Pharmacognosy, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 
      210009, China.
FAU - Qi, Lian-Wen
AU  - Qi LW
FAU - Li, Ping
AU  - Li P
FAU - Bao, Kang-De
AU  - Bao KD
FAU - Yan, Xiao-Wei
AU  - Yan XW
FAU - Yi, Ling
AU  - Yi L
FAU - Li, Chang-Yin
AU  - Li CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080304
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Coumaric Acids)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Glucosides)
RN  - 0 (Isoflavones)
RN  - 0 (Plant Extracts)
RN  - 0 (Saponins)
RN  - 0 (Triterpenes)
RN  - 0 (calycosin-7-O-beta-D-glucoside)
RN  - 0 (danggui buxue decoction)
RN  - 3A592W8XKE (astragaloside A)
RN  - AVM951ZWST (ferulic acid)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Coumaric Acids/*blood
MH  - Drugs, Chinese Herbal
MH  - Glucosides/*blood
MH  - Isoflavones/*blood
MH  - Plant Extracts/*chemistry
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
MH  - Saponins/*blood
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Triterpenes/*blood
EDAT- 2008/03/19 09:00
MHDA- 2008/08/09 09:00
CRDT- 2008/03/19 09:00
PHST- 2007/12/04 00:00 [received]
PHST- 2008/02/17 00:00 [revised]
PHST- 2008/02/19 00:00 [accepted]
PHST- 2008/03/19 09:00 [pubmed]
PHST- 2008/08/09 09:00 [medline]
PHST- 2008/03/19 09:00 [entrez]
AID - S1570-0232(08)00132-3 [pii]
AID - 10.1016/j.jchromb.2008.02.024 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):99-105. doi: 
      10.1016/j.jchromb.2008.02.024. Epub 2008 Mar 4.