PMID- 18347803
OWN - NLM
STAT- MEDLINE
DCOM- 20080826
LR  - 20211020
IS  - 0179-1958 (Print)
IS  - 0179-1958 (Linking)
VI  - 23
IP  - 6
DP  - 2008 Jun
TI  - A local imbalance between MMP and TIMP may have an implication on the severity 
      and course of appendicitis.
PG  - 611-8
LID - 10.1007/s00384-008-0452-x [doi]
AB  - BACKGROUND: Matrix metalloproteinases (MMPs) and the tissue inhibitors of MMPs 
      (TIMPs) have been demonstrated to be involved in inflammatory conditions in the 
      intestine. The purpose of this study was to investigate whether the alterations 
      of the MMP/TIMP balance might reflect the course of the inflammatory process in 
      acute appendicitis and if the expression and localisation of MMPs and TIMP is 
      variable in the various clinical manifestations of appendicitis. MATERIALS AND 
      METHODS: The study comprises 40 patients (26 men and 14 women) having emergency 
      appendectomy and a control group constituting of 10 patients (5 men and 5 women) 
      having a hemicolectomy for other reasons. MMP and TIMP expressions were assessed 
      and compared in tissue specimens from phlegmonous (n = 15), gangrenous (n = 7), 
      perforated appendicitis (n = 11) and controls with noninflamed appendices (n = 
      10) by means of enzyme-linked immunosorbent assay technique. Localisation of the 
      enzymes was performed by immunohistochemistry. RESULTS: MMP-1 was significantly 
      higher in gangrenous and perforated appendicitis compared with phlegmonous 
      appendicitis and controls (p < 0.05) while MMP-2 was significantly lower in 
      gangrenous appendicitis compared with phlegmonous appendicitis and controls. 
      MMP-2 was also lower in perforated appendicitis when compared with controls (p < 
      0.01). Elevated expression of MMP-9 was demonstrated in all groups of 
      appendicitis compared with the controls (p < 0.001). CONCLUSIONS: MMP-9 is the 
      most abundantly expressed MMP of those investigated in inflamed appendix. We 
      postulate that a local imbalance between MMP-9 and TIMP-1 may trigger a 
      perforation. These results suggest that MMPs might be useful as biomarkers of 
      appendices prone to perforation.
FAU - Solberg, Anna
AU  - Solberg A
AD  - Department of Surgery, Colorectal Unit, Sahlgrenska University Hospital/Ostra, 
      Gothenburg University, Gothenburg, Sweden. anna.solberg@surgery.gu.se
FAU - Holmdahl, Lena
AU  - Holmdahl L
FAU - Falk, Peter
AU  - Falk P
FAU - Palmgren, Ingrid
AU  - Palmgren I
FAU - Ivarsson, Marie-Louise
AU  - Ivarsson ML
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080318
PL  - Germany
TA  - Int J Colorectal Dis
JT  - International journal of colorectal disease
JID - 8607899
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Appendectomy/adverse effects
MH  - Appendicitis/*metabolism/pathology/surgery
MH  - Appendix/*metabolism
MH  - Emergencies
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinases/*metabolism
MH  - Middle Aged
MH  - Postoperative Complications
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
EDAT- 2008/03/19 09:00
MHDA- 2008/08/30 09:00
CRDT- 2008/03/19 09:00
PHST- 2008/01/23 00:00 [accepted]
PHST- 2008/03/19 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/03/19 09:00 [entrez]
AID - 10.1007/s00384-008-0452-x [doi]
PST - ppublish
SO  - Int J Colorectal Dis. 2008 Jun;23(6):611-8. doi: 10.1007/s00384-008-0452-x. Epub 
      2008 Mar 18.