PMID- 1834853
OWN - NLM
STAT- MEDLINE
DCOM- 19911206
LR  - 20190828
IS  - 0022-2828 (Print)
IS  - 0022-2828 (Linking)
VI  - 23
IP  - 4
DP  - 1991 Apr
TI  - Effects of low carbohydrate provision on isomyosin expression in the 
      isoproterenol stressed rat heart.
PG  - 453-60
AB  - While previous findings suggest that increased carbohydrate (CHO) provision 
      and/or utilization can be involved in the up regulation of V1 cardiac isomyosin 
      expression in both the insulin deficient and the semi-starved rat, little is 
      known about the role of CHO provision and/or utilization in regulating isomyosin 
      expression in normal animals. This study was undertaken to ascertain whether a 
      limited carbohydrate provision combined with repeated episodes of an increased 
      cardiac energy demand can induce a down regulation of the V1 cardiac myosin 
      inoenzyme in normal, young adult rats, i.e., a response similar to that seen in 
      insulin deficient/food restricted rats. Animals were assigned to one of three 
      major groups according to diet and/or drug treatment: (1) a mixed-diet control 
      group; (2) a low-carbohydrate (LC)/high-fat-diet group; and (3) a 
      low-carbohydrate-diet group treated with 3-mercaptopicolinic acid (MPA), an 
      inhibitor of gluconeogenesis. In each of the above groups, subgroups of animals 
      were metabolically challenged with daily isoproterenol injections (0.2 mg/kg/day) 
      sufficient to markedly elevate cardiac work for at least 3 h. Four weeks of 
      treatment reduced myofibrillar calcium activated ATPase activity by 17% (P less 
      than 0.05) in the LC-diet-fed group treated with both isoproterenol and MPA. No 
      change in myofibril ATPase was observed in any of the other experimental groups 
      relative to the mixed diet control group. Isomyosin profile was not changed in 
      any of the experimental groups. Tissue glycogen and plasma glucose and free fatty 
      acid analyses provided indirect evidence of an increased utilization of fat for 
      energy provision, especially in the low-carbohydrate-diet groups treated with 
      MPA.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Haddad, F
AU  - Haddad F
AD  - Department of Physiology and Biophysics, University of California, Irvine 92717.
FAU - Baldwin, K M
AU  - Baldwin KM
LA  - eng
GR  - R01 HL-38819/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Cell Cardiol
JT  - Journal of molecular and cellular cardiology
JID - 0262322
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 9005-79-2 (Glycogen)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.1 (Myosins)
RN  - IY9XDZ35W2 (Glucose)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Adenosine Triphosphatases/metabolism
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Body Weight
MH  - Dietary Carbohydrates/*administration & dosage
MH  - Female
MH  - Glucose/metabolism
MH  - Glycogen/metabolism
MH  - Heart/anatomy & histology/drug effects
MH  - Isoproterenol/*pharmacology
MH  - Liver/metabolism
MH  - Myocardium/*metabolism
MH  - Myosins/*metabolism
MH  - Organ Size
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 0022-2828(91)90169-M [pii]
AID - 10.1016/0022-2828(91)90169-m [doi]
PST - ppublish
SO  - J Mol Cell Cardiol. 1991 Apr;23(4):453-60. doi: 10.1016/0022-2828(91)90169-m.