PMID- 18350294
OWN - NLM
STAT- MEDLINE
DCOM- 20080918
LR  - 20080523
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Linking)
VI  - 87
IP  - 7
DP  - 2008 Jul
TI  - Chromosome 20 deletions in myelodysplastic syndromes and 
      Philadelphia-chromosome-negative myeloproliferative disorders: characterization 
      by molecular cytogenetics of commonly deleted and retained regions.
PG  - 537-44
LID - 10.1007/s00277-008-0462-3 [doi]
AB  - Deletion of the long arm of chromosome 20 is a recurrent abnormality observed in 
      myelodysplastic syndromes (MDS) and in Philadelphia-chromosome-negative 
      myeloproliferative disorders (MPD). Our objective was to characterize the 
      deletion size among 38 MDS and MPD patients using fluorescence in situ 
      hybridization (FISH) with bacterial artificial chromosome (BAC) probes and to 
      define commonly deleted and retained regions on chromosome 20. Patients were 
      distributed in three groups according to the World Health Organization 
      classification: MDS (22 patients), MPD (12 patients) and 
      myelodysplastic/myeloproliferative diseases (four patients). FISH with 
      centromeric, subtelomeric, and unique sequence probes was performed to 
      characterize the deletion whereas its size was delineated using BAC clones. All 
      38 deletions were found to be interstitial. A commonly deleted region was 
      identified for each of the three groups; it varied from 6.62 to 10.4 Mb and 
      showed considerable overlapping. Two commonly retained regions (CRR), also 
      showing overlapping, were identified in all three groups, one in the centromeric 
      region, the other in the telomeric region. The deletion size is highly variable, 
      with no apparent recurrent breakpoint. The deletion may result in the loss of one 
      or several tumor suppressor genes but the target genes remain unknown. Loss of 
      genes plays an important part in the myeloid leukemic process associated with 
      del(20q). However, genes located in the retained chromosomal regions may also 
      play a role in the oncogenetic mechanisms.
FAU - Douet-Guilbert, Nathalie
AU  - Douet-Guilbert N
AD  - Laboratoire d'Histologie, Embryologie et Cytogenetique, Faculte de Medecine et 
      des Sciences de la Sante, Universite de Bretagne Occidentale, Brest, France.
FAU - Basinko, Audrey
AU  - Basinko A
FAU - Morel, Frederic
AU  - Morel F
FAU - Le Bris, Marie-Josee
AU  - Le Bris MJ
FAU - Ugo, Valerie
AU  - Ugo V
FAU - Morice, Patrick
AU  - Morice P
FAU - Berthou, Christian
AU  - Berthou C
FAU - De Braekeleer, Marc
AU  - De Braekeleer M
LA  - eng
PT  - Journal Article
DEP - 20080319
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Banding
MH  - *Chromosome Deletion
MH  - Chromosomes, Artificial, Bacterial
MH  - Chromosomes, Human, Pair 20/genetics/*ultrastructure
MH  - Cohort Studies
MH  - Female
MH  - Gene Deletion
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative/genetics
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - Myeloproliferative Disorders/*genetics
MH  - Philadelphia Chromosome
EDAT- 2008/03/20 09:00
MHDA- 2008/09/19 09:00
CRDT- 2008/03/20 09:00
PHST- 2007/10/24 00:00 [received]
PHST- 2008/02/04 00:00 [accepted]
PHST- 2008/03/20 09:00 [pubmed]
PHST- 2008/09/19 09:00 [medline]
PHST- 2008/03/20 09:00 [entrez]
AID - 10.1007/s00277-008-0462-3 [doi]
PST - ppublish
SO  - Ann Hematol. 2008 Jul;87(7):537-44. doi: 10.1007/s00277-008-0462-3. Epub 2008 Mar 
      19.