PMID- 18352828
OWN - NLM
STAT- MEDLINE
DCOM- 20080623
LR  - 20181201
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 14
IP  - 4
DP  - 2008 Apr
TI  - A three-dimensional tubular scaffold that modulates the osteogenic and 
      vasculogenic differentiation of rat bone marrow stromal cells.
PG  - 491-504
LID - 10.1089/tea.2007.0235 [doi]
AB  - Bone marrow stromal cells (BMSCs) or mesenchymal stem cells (MSCs) are a 
      heterogeneous population of cells that are multipotent. When rat BMSCs were 
      seeded onto a 3-dimensional (3-D) tubular scaffold engineered from aligned type I 
      collagen strands and cultured in osteogenic medium, they simultaneously matured 
      and differentiated into osteoblastic and vascular cell lineages. In addition, 
      these osteoblasts produced mineralized matricellular deposits. BMSCs were seeded 
      at a density of 2 x 10(6) cells/15 mm tube and cultured in basal or osteogenic 
      medium for 3, 6, and 9 days. These cells were subsequently processed for 
      real-time reverse-transcriptase polymerase chain reaction (RT-qPCR), 
      immunohistochemical, cytochemical, and biochemical analyses. Immunolocalization 
      of lineage-specific proteins was visualized using confocal microscopy. In the 
      present study, the expression pattern of key osteogenic markers significantly 
      differed in response to basal and osteogenic media. Alkaline phosphatase activity 
      and calcium content increased significantly over the observed period of time in 
      osteogenic medium. The observed up-regulation of transcripts coding for 
      osteoblastic phenotypic markers is reminiscent of in vivo expression patterns. 
      Abundant sheets of Pecam (CD31) -, Flk-1 (vascular endothelial growth factor 
      receptor-2) -, CD34-, tomato lectin-, and alpha-smooth muscle actin-positive 
      cells were observed in these tube cultures. Moreover, nascent capillary-like 
      vessels were also seen amid the osteoblasts in osteogenic cultures. Our 3-D 
      culture system augmented the maturation and differentiation of BMSCs into 
      osteoblasts. Thus, our in vitro model provides an excellent opportunity to study 
      the concurrent temporal and spatial regulation of osteogenesis and vasculogenesis 
      during bone development.
FAU - Valarmathi, Mani T
AU  - Valarmathi MT
AD  - Department of Cell and Developmental Biology and Anatomy, School of Medicine, 
      University of South Carolina, Columbia, South Carolina 29209, USA. 
      valarmathi@gw.med.sc.edu
FAU - Yost, Michael J
AU  - Yost MJ
FAU - Goodwin, Richard L
AU  - Goodwin RL
FAU - Potts, Jay D
AU  - Potts JD
LA  - eng
GR  - R01 HL072958-01A1/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Collagen Type I)
RN  - 0 (Culture Media)
RN  - 0 (Extracellular Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - Calcification, Physiologic
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Collagen Type I
MH  - Culture Media
MH  - Endothelial Cells/*cytology
MH  - Extracellular Matrix Proteins
MH  - Male
MH  - Mesenchymal Stem Cells/cytology
MH  - *Neovascularization, Physiologic
MH  - Osteoblasts/*cytology
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells
MH  - *Tissue Scaffolds
EDAT- 2008/03/21 09:00
MHDA- 2008/06/24 09:00
CRDT- 2008/03/21 09:00
PHST- 2008/03/21 09:00 [pubmed]
PHST- 2008/06/24 09:00 [medline]
PHST- 2008/03/21 09:00 [entrez]
AID - 10.1089/tea.2007.0235 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2008 Apr;14(4):491-504. doi: 10.1089/tea.2007.0235.