PMID- 18353280
OWN - NLM
STAT- MEDLINE
DCOM- 20080730
LR  - 20080414
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1206
DP  - 2008 Apr 24
TI  - Expression changes of multiple brain-derived neurotrophic factor transcripts in 
      selective spinal nerve ligation model and complete Freund's adjuvant model.
PG  - 13-9
LID - 10.1016/j.brainres.2007.12.004 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) expression changes in the dorsal root 
      ganglion (DRG) and spinal cord in some pain models. Recently, rat BDNF 
      transcripts containing novel 5' untranslated exons were identified and 
      characterized, and a new numbering system for rat BDNF exons was introduced. We 
      examined the expression profiles of these novel BDNF transcripts in bilateral 
      L4/5 DRGs in an L5-selective spinal nerve ligation (SSNL) model and bilateral L5 
      DRGs in a complete Freund's adjuvant (CFA) model of rats. L5SSNL increased 
      significantly (P<0.05) the expression of total BDNF mRNA and exon I, IIA, IIB, 
      IIC, III, IV, VI, and IXA transcripts in ipsilateral L4 DRG. Although expression 
      of total BDNF mRNA remained unchanged in ipsilateral L5 DRG in the L5SSNL model, 
      expression of exon I transcript increased significantly (P<0.05) and that of exon 
      IV transcript decreased significantly (P<0.05). The expression profiles of the 
      variant exons in ipsilateral L4 DRG of the L5SSNL model were quite similar to 
      those in ipsilateral DRG of the CFA model, and exon I transcript was the most 
      common BDNF mRNA in these DRGs. Although L5SSNL increased significantly (P<0.05) 
      the expression of total BDNF mRNA and exon IIC and IXA transcripts in the 
      contralateral L4/5 DRGs, CFA treatment did not alter the expression of total BDNF 
      mRNA or specific transcripts in the contralateral DRGs. These findings suggest 
      that exon I plays an important role in the increase in BDNF expression in 
      ipsilateral DRGs regardless of condition.
FAU - Kobayashi, Hiroyuki
AU  - Kobayashi H
AD  - Department of Anesthesiology and Resuscitology, Okayama University Graduate 
      School Medicine, Dentistry, and Pharmaceutical Sciences, 2-5-1, Shikata-cho, 
      Okayama City 700-8558, Japan.
FAU - Yokoyama, Masataka
AU  - Yokoyama M
FAU - Matsuoka, Yoshikazu
AU  - Matsuoka Y
FAU - Omori, Megumi
AU  - Omori M
FAU - Itano, Yoshitaro
AU  - Itano Y
FAU - Kaku, Rhuji
AU  - Kaku R
FAU - Morita, Kiyoshi
AU  - Morita K
FAU - Ichikawa, Hiroyuki
AU  - Ichikawa H
LA  - eng
PT  - Journal Article
DEP - 20071208
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Exons/genetics
MH  - Freund's Adjuvant
MH  - Ganglia, Spinal/*metabolism
MH  - Ligation
MH  - Lumbar Vertebrae
MH  - Male
MH  - Mononeuropathies/chemically induced/*metabolism
MH  - Pain/*metabolism
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Spinal Cord/*metabolism
MH  - Spinal Nerves/metabolism/physiopathology
EDAT- 2008/03/21 09:00
MHDA- 2008/07/31 09:00
CRDT- 2008/03/21 09:00
PHST- 2007/09/12 00:00 [received]
PHST- 2007/11/10 00:00 [revised]
PHST- 2007/12/02 00:00 [accepted]
PHST- 2008/03/21 09:00 [pubmed]
PHST- 2008/07/31 09:00 [medline]
PHST- 2008/03/21 09:00 [entrez]
AID - S0006-8993(07)02873-9 [pii]
AID - 10.1016/j.brainres.2007.12.004 [doi]
PST - ppublish
SO  - Brain Res. 2008 Apr 24;1206:13-9. doi: 10.1016/j.brainres.2007.12.004. Epub 2007 
      Dec 8.