PMID- 18353283
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20151119
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1205
DP  - 2008 Apr 18
TI  - Transplantation of human amniotic cells exerts neuroprotection in MPTP-induced 
      Parkinson disease mice.
PG  - 108-15
LID - 10.1016/j.brainres.2008.02.040 [doi]
AB  - To determine the survival and differentiation of cultured Human amniotic cells 
      (HACs) upon transplantation into the brain of 
      1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced Parkinson disease (PD) 
      mice. Mouse model of PD was established with injections of MPTP (15 mg/kg, 
      fourth, 2 h interval). After being labeled with PKH26, HACs isolated from human 
      were transplanted into the striatum of PD mice. Immunohistochemistry was 
      performed to evaluate the toxicity of MPTP in the substantia nigra, graft 
      survival and endogenous neurogenesis. Brain-derived neurotrophic factor (BDNF) 
      and glial cell line-derived neurotrophic factor (GDNF) level in the striatum were 
      tested by ELISA. Our results showed that cultured HACs can express the marker of 
      neural progenitor cells and differentiate into neuron, dopaminergic neuron, 
      astrocyte and oligodendrocyte. TH-positive neural cells were significantly 
      reduced in the substantia nigra in the model mice, whereas which increased in 
      transplantation mice. Immunohistology results showed that transplanted HACs 
      survived and migrated in the brain of PD model mouse, though no morphological 
      integration was observed. BrdU-positive cells in the Subventricular zone (SVZ) 
      and neurotrophins of the striatum increased in the transplantation mice. The 
      results suggested that transplanted HACs could survive and promote the endogenous 
      neurogenesis of mice, which maybe related to the increased level of neurotrophins 
      of the striatum.
FAU - Kong, Xiang-Ying
AU  - Kong XY
AD  - Department of Pharmacology, School of Basic Medicine, Peking Union Medical 
      College and Institute of Basic Medical Science, Chinese Academy of Medical 
      Sciences, Beijing, 100005 China.
FAU - Cai, Zhe
AU  - Cai Z
FAU - Pan, Lin
AU  - Pan L
FAU - Zhang, Lan
AU  - Zhang L
FAU - Shu, Jun
AU  - Shu J
FAU - Dong, Yi-Long
AU  - Dong YL
FAU - Yang, Nan
AU  - Yang N
FAU - Li, Qing
AU  - Li Q
FAU - Huang, Xiao-Jie
AU  - Huang XJ
FAU - Zuo, Ping-Ping
AU  - Zuo PP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080226
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Antimetabolites)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Amnion/*cytology
MH  - Animals
MH  - Antimetabolites
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Bromodeoxyuridine
MH  - Cell Survival/physiology
MH  - Cell Transplantation/*physiology
MH  - Cerebral Ventricles/cytology/physiology
MH  - Dopamine/physiology
MH  - Fluorescent Antibody Technique
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - MPTP Poisoning/pathology/*prevention & control
MH  - Mice
MH  - Motor Activity/physiology
MH  - Neostriatum/cytology/metabolism/physiology
MH  - Nerve Growth Factors/metabolism
MH  - Postural Balance/physiology
MH  - Tissue Fixation
MH  - Transplantation, Heterologous
MH  - Tyrosine 3-Monooxygenase/metabolism
EDAT- 2008/03/21 09:00
MHDA- 2008/07/23 09:00
CRDT- 2008/03/21 09:00
PHST- 2008/01/18 00:00 [received]
PHST- 2008/02/07 00:00 [accepted]
PHST- 2008/03/21 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/03/21 09:00 [entrez]
AID - S0006-8993(08)00374-0 [pii]
AID - 10.1016/j.brainres.2008.02.040 [doi]
PST - ppublish
SO  - Brain Res. 2008 Apr 18;1205:108-15. doi: 10.1016/j.brainres.2008.02.040. Epub 
      2008 Feb 26.