PMID- 18353310
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20220321
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 584
IP  - 2-3
DP  - 2008 Apr 28
TI  - Facilitating action of asiaticoside at low doses on burn wound repair and its 
      mechanism.
PG  - 415-23
LID - 10.1016/j.ejphar.2008.02.036 [doi]
AB  - Some reports published from 1967 to 1999 describe the use of ointments containing 
      high doses (0.1 to 0.2%, w/w) C. asiataica herb extracts to enhance wound repair. 
      Lower doses at which burn wound repair is enhanced by such topical applications 
      have not been established yet. We found that the application of asiaticoside at 
      low doses of 10(-8) to 10(-12)% (w/w) facilitated burn wound repair. To clarify 
      the accelerating mechanisms of asiaticoside on burn wound repair, we examined the 
      effects of asiaticoside on the levels of various cytokines produced at the site 
      of the burn wound. The topical application of a low dose (10 pg, 1 ng, or 100 
      ng/wound area) of asiaticoside increased monocyte chemoattractant protein-1 
      (MCP-1), vascular endothelial growth factor (VEGF), and interleukin (IL)-1beta 
      levels in burn wound exudates. Asiaticoside (10 pg to 100 ng/ml) enhanced MCP-1 
      production in HaCaT cells, but it had no direct effect on VEGF production. 
      Furthermore, asiaticoside (10 pg to 100 ng/ml) increased the IL-1beta production 
      in THP-1 macrophages with MCP-1, but it had no effect on IL-1beta production 
      without MCP-1 or with lipopolysaccharide (LPS). These findings suggest that the 
      enhancement of burn wound healing by asiaticoside might be due to the promotion 
      of angiogenesis during skin wound repair as a result of the stimulation of VEGF 
      production caused by the increase in MCP-1 expression in keratinocytes and the 
      increase in IL-1beta expression in macrophages induced cooperatively by 
      asiaticoside plus MCP-1.
FAU - Kimura, Yoshiyuki
AU  - Kimura Y
AD  - Division of Biochemical Pharmacology, Department of Basic Medical Research, Ehime 
      University Graduate School of Medicine, Shitsukawa, Toon City, 791-0295, Japan. 
      yokim@m.ehime-u.ac.jp
FAU - Sumiyoshi, Maho
AU  - Sumiyoshi M
FAU - Samukawa, Kei-Ichi
AU  - Samukawa K
FAU - Satake, Noriko
AU  - Satake N
FAU - Sakanaka, Masahiro
AU  - Sakanaka M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080221
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Centella asiatica extract)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Dermatologic Agents)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Plant Extracts)
RN  - 0 (Triterpenes)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, mouse)
RN  - PKO39VY215 (asiaticoside)
SB  - IM
MH  - Administration, Cutaneous
MH  - Animals
MH  - Burns/*drug therapy/immunology/metabolism/physiopathology
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Centella
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*metabolism
MH  - Dermatologic Agents/*pharmacology/therapeutic use
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Exudates and Transudates/drug effects/metabolism
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Keratinocytes/drug effects/immunology
MH  - Macrophages/drug effects/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neovascularization, Physiologic/drug effects
MH  - Plant Extracts
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Triterpenes/administration & dosage/*pharmacology
MH  - Vascular Endothelial Growth Factor A/metabolism
MH  - Wound Healing/*drug effects/immunology
EDAT- 2008/03/21 09:00
MHDA- 2008/07/02 09:00
CRDT- 2008/03/21 09:00
PHST- 2007/05/25 00:00 [received]
PHST- 2008/01/31 00:00 [revised]
PHST- 2008/02/13 00:00 [accepted]
PHST- 2008/03/21 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2008/03/21 09:00 [entrez]
AID - S0014-2999(08)00167-2 [pii]
AID - 10.1016/j.ejphar.2008.02.036 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2008 Apr 28;584(2-3):415-23. doi: 10.1016/j.ejphar.2008.02.036. 
      Epub 2008 Feb 21.