PMID- 18353886
OWN - NLM
STAT- MEDLINE
DCOM- 20080618
LR  - 20200930
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 294
IP  - 5
DP  - 2008 May
TI  - Inflammatory gene changes associated with the repeated-bout effect.
PG  - R1628-37
LID - 10.1152/ajpregu.00853.2007 [doi]
AB  - This study proposed that attenuated expression of inflammatory factors is an 
      underlying mechanism driving the repeated-bout effect (rapid adaptation to 
      eccentric exercise). We investigated changes in mRNA levels and protein 
      localization of inflammatory genes after two bouts of muscle-lengthening 
      exercise. Seven male subjects performed two bouts of lower body exercise 
      (separated by 4 wk) in which one leg performed 300 eccentric-concentric actions, 
      and the contralateral leg performed 300 concentric actions only. Vastus lateralis 
      biopsies were collected at 6 h, and strength was assessed at baseline and at 0, 
      3, and 5 days after exercise. mRNA levels were measured via semiquantitative 
      RT-PCR for the following genes: CYR61, HSP40, HSP70, IL1R1, TCF8, ZFP36, CEBPD, 
      and MCP1. Muscle functional adaptation was demonstrated via attenuated strength 
      loss (16% less, P = 0.04) at 5 days after bout 2 compared with bout 1 in the 
      eccentrically exercised leg. mRNA expression of three of the eight genes tested 
      was significantly elevated in the eccentrically exercised leg from bout 1 to bout 
      2 (+3.9-fold for ZFP36, +2.3-fold for CEBPD, and +2.6-fold for MCP1), while all 
      eight mRNA levels were unaffected by bout in the concentrically exercised leg. 
      Immunohistochemistry further localized the protein of one of the elevated factors 
      [monocyte chemoattractant protein-1 (MCP1)] within the tissue. MCP1 colocalized 
      with resident macrophage and satellite cell populations, suggesting that 
      alterations in cytokine signaling between these cell populations may play a role 
      in muscle adaptation to exercise. Contrary to our hypothesis, several 
      inflammatory genes were transcriptionally upregulated (rather than attenuated) 
      after a repeated exercise bout, potentially indicating a role for these genes in 
      the adaptation process.
FAU - Hubal, Monica J
AU  - Hubal MJ
AD  - Department of Kinesiology, University of Massachusetts, Amherst, MA, USA. 
      mhubal@cnmcresearch.org
FAU - Chen, Trevor C
AU  - Chen TC
FAU - Thompson, Paul D
AU  - Thompson PD
FAU - Clarkson, Priscilla M
AU  - Clarkson PM
LA  - eng
GR  - 1 F31 AR 52312-1/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080319
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA, Complementary)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - DNA, Complementary/biosynthesis/genetics
MH  - Data Interpretation, Statistical
MH  - Exercise/*physiology
MH  - Gene Expression/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation/*genetics
MH  - Inflammation Mediators/metabolism
MH  - Isometric Contraction/physiology
MH  - Male
MH  - Muscle Proteins/biosynthesis/genetics
MH  - Muscle Strength/physiology
MH  - Muscle, Skeletal/metabolism/physiology
MH  - Physical Fitness/physiology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/03/21 09:00
MHDA- 2008/06/19 09:00
CRDT- 2008/03/21 09:00
PHST- 2008/03/21 09:00 [pubmed]
PHST- 2008/06/19 09:00 [medline]
PHST- 2008/03/21 09:00 [entrez]
AID - 00853.2007 [pii]
AID - 10.1152/ajpregu.00853.2007 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2008 May;294(5):R1628-37. doi: 
      10.1152/ajpregu.00853.2007. Epub 2008 Mar 19.