PMID- 18355242
OWN - NLM
STAT- MEDLINE
DCOM- 20081010
LR  - 20220309
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 125
IP  - 1
DP  - 2008 Sep
TI  - Serum-derived exosomes from antigen-fed mice prevent allergic sensitization in a 
      model of allergic asthma.
PG  - 21-7
LID - 10.1111/j.1365-2567.2008.02812.x [doi]
AB  - Oral tolerance is an active process that starts with sampling of luminal antigens 
      by the intestinal epithelial cells (IEC), followed by processing and assembly 
      with major histocompatibility complex class II and subsequently a release of 
      tolerogenic exosomes (tolerosomes) from the IEC. We have previously shown that 
      tolerosomes can be isolated from serum shortly after an antigen feed, and will 
      potently transfer antigen-specific tolerance to naive recipients. Here we study 
      the capacity of the tolerosomes to protect against allergic sensitization in a 
      mouse model of allergic asthma. Serum or isolated serum exosomes from tolerized 
      BALB/c donor mice were transferred to syngeneic recipients followed by 
      sensitization and intranasal exposure to ovalbumin (OVA). Blood, bronchoalveolar 
      lavage (BAL) and lymph nodes were sampled 24 hr after the final exposure. The 
      number of eosinophils was counted in BAL fluid and the levels of immunoglobulin E 
      (IgE) and OVA-specific IgE were measured in serum. Mediastinal and coeliac lymph 
      nodes were analysed by flow cytometry. The animals receiving serum from OVA-fed 
      mice displayed significantly lower numbers of airway eosinophils and lower serum 
      levels of total IgE as well as of OVA-specific IgE compared with controls. 
      Moreover, the tolerant animals showed a significantly higher frequency of 
      activated T cells with a regulatory phenotype in both mediastinal and coeliac 
      lymph nodes. The results show that serum or isolated serum exosomes obtained from 
      OVA-fed mice and administered intraperitoneally to naive recipient mice abrogated 
      allergic sensitization in the recipients.
FAU - Almqvist, Nina
AU  - Almqvist N
AD  - Department of Rheumatology and Inflammation Research, Goteborg University, 
      Goteborg, Sweden. nina.almqvist@rheuma.gu.se
FAU - Lonnqvist, Anna
AU  - Lonnqvist A
FAU - Hultkrantz, Susanne
AU  - Hultkrantz S
FAU - Rask, Carola
AU  - Rask C
FAU - Telemo, Esbjorn
AU  - Telemo E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080319
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Immune Sera)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/immunology
MH  - Animals
MH  - Asthma/immunology/*prevention & control
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Cytoplasmic Vesicles/*immunology
MH  - Disease Models, Animal
MH  - Immune Sera/immunology
MH  - Immune Tolerance
MH  - Immunity, Mucosal
MH  - Immunoglobulin E/blood
MH  - Intestinal Mucosa/immunology
MH  - Liver/immunology
MH  - Lung/immunology
MH  - Lymph Nodes/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - Passive Cutaneous Anaphylaxis/immunology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - T-Lymphocytes, Regulatory/immunology
PMC - PMC2526256
EDAT- 2008/03/22 09:00
MHDA- 2008/10/11 09:00
PMCR- 2009/09/01
CRDT- 2008/03/22 09:00
PHST- 2008/03/22 09:00 [pubmed]
PHST- 2008/10/11 09:00 [medline]
PHST- 2008/03/22 09:00 [entrez]
PHST- 2009/09/01 00:00 [pmc-release]
AID - IMM2812 [pii]
AID - 10.1111/j.1365-2567.2008.02812.x [doi]
PST - ppublish
SO  - Immunology. 2008 Sep;125(1):21-7. doi: 10.1111/j.1365-2567.2008.02812.x. Epub 
      2008 Mar 19.