PMID- 18355932
OWN - NLM
STAT- MEDLINE
DCOM- 20080612
LR  - 20211020
IS  - 0166-0934 (Print)
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 149
IP  - 2
DP  - 2008 May
TI  - Rapid detection of porcine circovirus type 2 by loop-mediated isothermal 
      amplification.
PG  - 264-8
LID - 10.1016/j.jviromet.2008.01.023 [doi]
AB  - A method of loop-mediated isothermal amplification (LAMP) was employed to develop 
      a rapid and simple detection system for porcine circovirus type 2 (PCV2). The 
      amplification could be finished in 60 min under isothermal condition at 64 
      degrees C by employing a set of four primers targeting the cap gene of PCV2. The 
      LAMP assay showed higher sensitivity than the conventional PCR, with a detection 
      limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity 
      was observed from the samples of other related viruses including porcine 
      circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus 
      (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The 
      detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared 
      greater than that of the PCR method. The LAMP assay reported can provide a rapid 
      yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection 
      due to ease of operation and the requirement of only a regular water bath or heat 
      block for the reaction.
FAU - Chen, Hao-Tai
AU  - Chen HT
AD  - Key Laboratory of Animal Virology of Ministry of Agriculture, State Key 
      Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
FAU - Zhang, Jie
AU  - Zhang J
FAU - Sun, De-Hui
AU  - Sun DH
FAU - Chu, Yue-Feng
AU  - Chu YF
FAU - Cai, Xue-Peng
AU  - Cai XP
FAU - Liu, Xiang-Tao
AU  - Liu XT
FAU - Luo, Xue-Nong
AU  - Luo XN
FAU - Liu, Qing
AU  - Liu Q
FAU - Liu, Yong-Sheng
AU  - Liu YS
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080319
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Circoviridae Infections/*diagnosis
MH  - Circovirus/genetics/*isolation & purification
MH  - DNA Primers/genetics
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis
MH  - Temperature
MH  - Time Factors
PMC - PMC7112855
EDAT- 2008/03/22 09:00
MHDA- 2008/06/13 09:00
PMCR- 2008/03/19
CRDT- 2008/03/22 09:00
PHST- 2007/07/05 00:00 [received]
PHST- 2008/01/18 00:00 [revised]
PHST- 2008/01/22 00:00 [accepted]
PHST- 2008/03/22 09:00 [pubmed]
PHST- 2008/06/13 09:00 [medline]
PHST- 2008/03/22 09:00 [entrez]
PHST- 2008/03/19 00:00 [pmc-release]
AID - S0166-0934(08)00040-2 [pii]
AID - 10.1016/j.jviromet.2008.01.023 [doi]
PST - ppublish
SO  - J Virol Methods. 2008 May;149(2):264-8. doi: 10.1016/j.jviromet.2008.01.023. Epub 
      2008 Mar 19.