PMID- 18357408
OWN - NLM
STAT- MEDLINE
DCOM- 20080703
LR  - 20080418
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 153
IP  - 5
DP  - 2008
TI  - Recovery of viable porcine reproductive and respiratory syndrome virus from an 
      infectious clone containing a partial deletion within the Nsp2-encoding region.
PG  - 899-907
LID - 10.1007/s00705-008-0064-3 [doi]
AB  - Non-structural protein 2 (Nsp2) of porcine reproductive and respiratory syndrome 
      virus (PRRSV) is the most variable region and postulated to play an important 
      role in cell and tissue tropism of PRRSV. To investigate the role of Nsp2 in the 
      viability and growth of PRRSV in cells in vitro, two cDNA clones were constructed 
      containing a deletion of 63 consecutive nucleotides (pWSK-DCBAd63) or 117 
      nucleotides (pWSK-DCBAd117) within the Nsp2-encoding region of PRRSV (BJ-4). The 
      clone pWSK-DCBAd63 was infectious and produced viable recombinant virus, whereas 
      clone pWSK-DCBAd117 could not be rescued. The rescued virus was able to induce 
      CPE typical of PRRSV on MARC-145 cells and was stably propagated during 
      sequential in vitro cell passages, like the virus recovered from the full-length 
      cDNA clone of PRRSV BJ-4. In comparison to the parental virus (BJ-4) and the 
      virus recovered from the full-length cDNA clone of the BJ-4 strain, the rescued 
      virus from pWSK-DCBAd63 exhibited enhanced growth kinetics, reaching the peak 
      progeny virus titer by 48 h postinfection. These observations suggest that the 
      Nsp2-encoding region is necessary for productive virus infection, and partial 
      deletion does not influence the viability and propagation of PRRSV in cell 
      culture, which may provide a way to insert a foreign gene into the viral genome 
      as a marker for differentiation.
FAU - Ran, Z G
AU  - Ran ZG
AD  - Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, 
      College of Veterinary Medicine and State Key Laboratory of Agrobiotechnolgy, 
      China Agricultural University, 10094 Beijing, People's Republic of China.
FAU - Chen, X Y
AU  - Chen XY
FAU - Guo, X
AU  - Guo X
FAU - Ge, X N
AU  - Ge XN
FAU - Yoon, K J
AU  - Yoon KJ
FAU - Yang, H C
AU  - Yang HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080321
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Clone Cells
MH  - DNA, Viral/genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/isolation & 
      purification/*pathogenicity
MH  - Sequence Deletion
MH  - Swine
MH  - Transcription, Genetic
MH  - Transfection
MH  - Viral Nonstructural Proteins/genetics
EDAT- 2008/03/22 09:00
MHDA- 2008/07/04 09:00
CRDT- 2008/03/22 09:00
PHST- 2007/05/09 00:00 [received]
PHST- 2008/01/22 00:00 [accepted]
PHST- 2008/03/22 09:00 [pubmed]
PHST- 2008/07/04 09:00 [medline]
PHST- 2008/03/22 09:00 [entrez]
AID - 10.1007/s00705-008-0064-3 [doi]
PST - ppublish
SO  - Arch Virol. 2008;153(5):899-907. doi: 10.1007/s00705-008-0064-3. Epub 2008 Mar 
      21.