PMID- 18357730
OWN - NLM
STAT- MEDLINE
DCOM- 20090810
LR  - 20080324
IS  - 0513-4870 (Print)
IS  - 0513-4870 (Linking)
VI  - 43
IP  - 1
DP  - 2008 Jan
TI  - [Effect of sophocarpine on HERG K+ channels].
PG  - 44-9
AB  - Human ether-a-go-go-related gene (HERG) encodes the rapid component of the 
      cardiac delayed rectifier K+ current, which has an important effect on both 
      proarrhythmia and antiarrhythmia. To investigate the effect of sophocarpine (SC) 
      on HERG channel stably expressing in human embryonic kidney-293 (HEK293) cells, 
      whole-cell patch-clamp technique was used to record HERG current and kinetic 
      curves. As the result, it was found that SC inhibited HERG current in a 
      concentration-dependent manner (10, 30, 100, and 300 micromol x L(-1)). At 0 mV, 
      10, 30, 100, and 300 micromol x L(-1) SC respectively inhibited IHERG by Istep ( 
      10.7 +/- 2.8)% , (11.3 +/- 5.5)% , (47.0 +/- 2.3)% and (53.7 +/- 2.5)% , and 
      Itail (1.1 +/- 3.0)%, (17.1 +/- 3.3)%, (32.7 +/- 1.9)% (P < 0.05, n = 12) and 
      (56.0 +/- 2.4)% (P < 0.05, n = 13). The time constants of inactivation, recovery 
      from inactivation and onset of inactivation were accelerated. SC did not change 
      other channel kinetics (activation and deactivation). It is concluded that SC 
      inhibited the transfected HERG channels by influencing the inactivation state, 
      which is the probable anti-arrhythmic mechanism.
FAU - Qi, Zhi-Ping
AU  - Qi ZP
AD  - Department of Pharmacology, Bio-pharmaceutical Key Laboratory of Heilongjiang 
      Province-Incubator of State Key Laboratory, Harbin Medical University, Harbin 
      150086, China.
FAU - Shi, Shan-Shan
AU  - Shi SS
FAU - Zhao, Xue-Ling
AU  - Zhao XL
FAU - Zhao, Wen-Xiao
AU  - Zhao WX
FAU - Bai, Yun-Long
AU  - Bai YL
FAU - Lu, Yan-Jie
AU  - Lu YJ
FAU - Li, Bao-Xin
AU  - Li BX
FAU - Yang, Bao-Feng
AU  - Yang BF
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yao Xue Xue Bao
JT  - Yao xue xue bao = Acta pharmaceutica Sinica
JID - 21710340R
RN  - 0 (Alkaloids)
RN  - 0 (Anti-Arrhythmia Agents)
RN  - 0 (Ether-A-Go-Go Potassium Channels)
RN  - 6483-15-4 (sophocarpine)
SB  - IM
MH  - Alkaloids/*pharmacology
MH  - Anti-Arrhythmia Agents/*pharmacology
MH  - Cell Line
MH  - Dose-Response Relationship, Drug
MH  - Ether-A-Go-Go Potassium Channels/*antagonists & inhibitors/metabolism/*physiology
MH  - Humans
MH  - Kidney/cytology
MH  - Kinetics
MH  - Membrane Potentials/drug effects
MH  - Patch-Clamp Techniques
MH  - Plants, Medicinal/chemistry
MH  - Sophora/chemistry
EDAT- 2008/03/25 09:00
MHDA- 2009/08/11 09:00
CRDT- 2008/03/25 09:00
PHST- 2008/03/25 09:00 [pubmed]
PHST- 2009/08/11 09:00 [medline]
PHST- 2008/03/25 09:00 [entrez]
PST - ppublish
SO  - Yao Xue Xue Bao. 2008 Jan;43(1):44-9.