PMID- 18357839
OWN - NLM
STAT- MEDLINE
DCOM- 20080424
LR  - 20191110
IS  - 0003-4894 (Print)
IS  - 0003-4894 (Linking)
VI  - 117
IP  - 2
DP  - 2008 Feb
TI  - Inflammatory factor profiles one hour following vocal fold injury.
PG  - 145-52
AB  - OBJECTIVES: Inflammatory factors are key mediators of wound healing processes 
      following injury, and their modulation may improve healing outcomes. The 
      objective of this study was to characterize in vivo inflammatory factor and 
      extracellular matrix (ECM) messenger RNA (mRNA) expression levels 1 hour after 
      vocal fold injury. METHODS: Five Sprague-Dawley rats were subjected to bilateral 
      vocal fold injury, 5 rats were reserved as uninjured controls, and 1 rat was 
      subjected to unilateral vocal fold injury and reserved for histology. Tissue was 
      harvested 1 hour after injury. Real-time reverse transcription-polymerase chain 
      reaction was performed to examine the mRNA expression profiles of inflammatory 
      factors nuclear factor kappa beta (NF-kappabeta), interferon gamma (IFN-gamma), 
      cyclooxygenase 2 (COX-2), transforming growth factor beta isoform 1 (TGF-beta1), 
      tumor necrosis factor alpha (TNF-alpha), and interleukin 1 beta (IL-1beta), as 
      well as ECM genes hyaluronic acid synthase (HAS) 1, HAS-2, procollagen 1, 
      procollagen 3, and elastin, in the injured samples compared with the uninjured 
      controls. RESULTS: Injury resulted in subepithelial bleeding throughout the vocal 
      fold. The COX-2, TNF-alpha, IL-1beta, and HAS-1 mRNA expression levels were 
      significantly up-regulated 1 hour after injury compared with the uninjured 
      controls. CONCLUSIONS: Inflammatory factor and ECM gene expression changes occur 
      in vocal fold wound sites as early as 1 hour after injury. These results should 
      inform future efforts to attenuate vocal fold scarring via the modulation of 
      inflammatory factors.
FAU - Welham, Nathan V
AU  - Welham NV
AD  - Division of Otolaryngology, Department of Surgery, University of Wisconsin School 
      of Medicine and Public Health, Madison, Wisconsin, USA.
FAU - Lim, Xinhong
AU  - Lim X
FAU - Tateya, Ichiro
AU  - Tateya I
FAU - Bless, Diane M
AU  - Bless DM
LA  - eng
GR  - R01 DC004428/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Ann Otol Rhinol Laryngol
JT  - The Annals of otology, rhinology, and laryngology
JID - 0407300
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Extracellular Matrix Proteins/biosynthesis/genetics
MH  - Gene Expression
MH  - *Inflammation Mediators
MH  - Male
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Vocal Cords/*injuries
EDAT- 2008/03/25 09:00
MHDA- 2008/04/25 09:00
CRDT- 2008/03/25 09:00
PHST- 2008/03/25 09:00 [pubmed]
PHST- 2008/04/25 09:00 [medline]
PHST- 2008/03/25 09:00 [entrez]
AID - 10.1177/000348940811700213 [doi]
PST - ppublish
SO  - Ann Otol Rhinol Laryngol. 2008 Feb;117(2):145-52. doi: 
      10.1177/000348940811700213.