PMID- 18357958
OWN - NLM
STAT- MEDLINE
DCOM- 20080520
LR  - 20190917
IS  - 0148-5016 (Print)
IS  - 0148-5016 (Linking)
VI  - 53
IP  - 6
DP  - 2007 Nov-Dec
TI  - Human spermatozoa ultrastructure assessment in the infertility treatment by 
      assisted reproduction technique.
PG  - 297-302
AB  - The aim of this study was to prospectively investigate the spermatozoa 
      ultrastructure in relation to the results of in vitro fertilization-embryo 
      transer (IVF-ET). Forty-nine consecutive couples admitted for IVF-ET were 
      prospectively evaluated for electron microscopic spermatozoa morphology and the 
      outcome of IVF-ET. Thirty-four couples revealed successful fertilization, defined 
      as presence of two pronuclei 14-16 hours after spermatozoa administration, while 
      the remaining 15 formed the failure group. Spermatozoa fixed with 2.5% 
      glutaraldehyd and embedded in Spurr's resin were analyzed with JAM 100 S 
      transmission electron microscope (TEM) for the following ultrastructure 
      abnormalities: head deformity, cytoplasmic residues, chromatin condensation 
      failures, acrosomal alterations, neck defects, mid-piece defects, principal piece 
      and end-piece defects and immature forms. Successful IVF-ET couples revealed a 
      significantly higher percentage of normal spermatozoa utrastructure (32.0 +/- 
      13.1% versus 17.1 +/- 13.4%, p < 0.001). Failed IVF-ET couples represented a 
      significantly higher percentage of chromatin condensation failures (9.8 +/- 5.1% 
      versus 5.7 +/- 5.3%, p < 0.05) and tail defects (16.7 +/- 11.5% versus 7.2 +/- 
      7.2%, p < 0.001). A positive correlation between normal ultrastructure 
      spermatozoa percentage and fertilized oocytes percentage was found (r = 0.35, p < 
      0.05). Our data suggest that spermatozoa TEM findings correlate with IVF-ET 
      results. Ultrastructural estimation of spermatozoa can improve the diagnosis of 
      male fertility and may explain some reasons of failure in assisted reproduction 
      methods. We consider systematic TEM spermatozoa examination in cases with failed 
      IVF-ET prior to intracytoplasmic sperm injection (ICSI).
FAU - Malgorzata, Kotwicka
AU  - Malgorzata K
AD  - Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 
      5D, 60-806 Poznan, Poland. mkotwic@amp.edu.pl
FAU - Depa-Martynow, Magdalena
AU  - Depa-Martynow M
FAU - Butowska, Wanda
AU  - Butowska W
FAU - Filipiak, Krystyna
AU  - Filipiak K
FAU - Pawelczyk, Leszek
AU  - Pawelczyk L
FAU - Jedrzejczak, Piotr
AU  - Jedrzejczak P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Arch Androl
JT  - Archives of andrology
JID - 7806755
SB  - IM
MH  - Adult
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - *Infertility, Male
MH  - Male
MH  - *Reproductive Techniques, Assisted
MH  - Spermatozoa/*abnormalities/*ultrastructure
EDAT- 2008/03/25 09:00
MHDA- 2008/05/21 09:00
CRDT- 2008/03/25 09:00
PHST- 2008/03/25 09:00 [pubmed]
PHST- 2008/05/21 09:00 [medline]
PHST- 2008/03/25 09:00 [entrez]
AID - 10.1080/01485010701730708 [doi]
PST - ppublish
SO  - Arch Androl. 2007 Nov-Dec;53(6):297-302. doi: 10.1080/01485010701730708.