PMID- 18359185 OWN - NLM STAT- MEDLINE DCOM- 20080725 LR - 20161124 IS - 0887-2333 (Print) IS - 0887-2333 (Linking) VI - 22 IP - 4 DP - 2008 Jun TI - In vitro toxicity evaluation of diesel exhaust particles on human eosinophilic cell. PG - 988-94 LID - 10.1016/j.tiv.2008.02.004 [doi] AB - Diesel exhaust particles (DEPs), comprised mainly of particles less than 2.5 microm (PM 2.5) in aerodynamic diameter, have been assumed to enhance the response of asthma to allergen inhalation. Although eosinophilic infiltration is remarkable in the event of bronchial asthma induced by DEPs, the precise mechanisms leading to eosinophilia are unknown. To examine the effect of DEPs on eosinophils, we measured the cytokine products and activity of nuclear factor-kappa B (NF-kappa B) after addition of the proteasomal inhibitor MG132 in HL-60 clone 15 cells differentiated into eosinophils. We measured eotaxin-induced chemotaxis of cells and their activity of p38 mitogen-activated protein (MAP) kinase was analysed. Interleukin (IL)-8 and monocyte chemoattractant protein-1 (MCP-1) were increased markedly in DEPs-treated cells. The active form of NF-kappaB in cells treated with DEPs was increased, and this effect was significantly decreased by the administration of MG132. Cell migration in the presence of DEPs was significantly greater, and inhibited by adding N-acetyl l-cysteine. P38 MAP kinase activity was highly influenced by DEPs-treatment. DEPs induce MCP-1 and IL-8 production by up-regulating NF-kappa B activity, which is inhibited in the presence of an inhibitor of proteasomal degradation. DEP also promotes eotaxin-induced chemotaxis in a p38-dependent manner. FAU - Hirota, Ryoji AU - Hirota R AD - Department of Environmental Medicine, Kochi Medical School, Kohasu, Oko, Nakoku, Kochi 783-8505, Japan. hirotar@kochi-u.ac.jp FAU - Akimaru, Kunihiro AU - Akimaru K FAU - Nakamura, Hiroyuki AU - Nakamura H LA - eng PT - Journal Article DEP - 20080215 PL - England TA - Toxicol In Vitro JT - Toxicology in vitro : an international journal published in association with BIBRA JID - 8712158 RN - 0 (Chemokine CCL2) RN - 0 (Interleukin-8) RN - 0 (Leupeptins) RN - 0 (NF-kappa B) RN - 0 (Vehicle Emissions) RN - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) RN - RF1P63GW3K (benzyloxycarbonylleucyl-leucyl-leucine aldehyde) RN - WYQ7N0BPYC (Acetylcysteine) SB - IM MH - Acetylcysteine/pharmacology MH - Cell Movement/drug effects MH - Chemokine CCL2/drug effects/metabolism MH - Chemotaxis/drug effects MH - Clone Cells MH - Eosinophils/*drug effects/metabolism MH - HL-60 Cells MH - Humans MH - Interleukin-8/drug effects/metabolism MH - Leupeptins/pharmacology MH - NF-kappa B/*drug effects/metabolism MH - Up-Regulation/drug effects MH - Vehicle Emissions/*toxicity MH - p38 Mitogen-Activated Protein Kinases/*drug effects/metabolism EDAT- 2008/03/25 09:00 MHDA- 2008/07/26 09:00 CRDT- 2008/03/25 09:00 PHST- 2007/06/29 00:00 [received] PHST- 2007/12/25 00:00 [revised] PHST- 2008/02/08 00:00 [accepted] PHST- 2008/03/25 09:00 [pubmed] PHST- 2008/07/26 09:00 [medline] PHST- 2008/03/25 09:00 [entrez] AID - S0887-2333(08)00043-X [pii] AID - 10.1016/j.tiv.2008.02.004 [doi] PST - ppublish SO - Toxicol In Vitro. 2008 Jun;22(4):988-94. doi: 10.1016/j.tiv.2008.02.004. Epub 2008 Feb 15.