PMID- 18360785
OWN - NLM
STAT- MEDLINE
DCOM- 20080527
LR  - 20211020
IS  - 1436-6207 (Print)
IS  - 1436-6207 (Linking)
VI  - 47
IP  - 2
DP  - 2008 Mar
TI  - Eicosapentaenoic acid (EPA) increases cell viability and expression of 
      neurotrophin receptors in retinoic acid and brain-derived neurotrophic factor 
      differentiated SH-SY5Y cells.
PG  - 104-13
LID - 10.1007/s00394-008-0703-1 [doi]
AB  - BACKGROUND: The n-3 polyunsaturated fatty acid, eicosapentaenoic acid (EPA) has 
      been found to process neuroprotective effects. However, the exact cellular 
      mechanisms are not well understood. Brain-derived neurotrophic factor (BDNF) is 
      one of neurotrophins, which is involved in neuron differentiation, survival, and 
      synaptogenesis. AIM OF THE STUDY: In this study, the potential neuroprotective 
      effects of EPA, and its possible effects on BDNF and BDNF receptor expression 
      were investigated in SH-SY5Y cells. METHODS: Both undifferentiated and retinoic 
      acid (RA)-BDNF differentiated SH-SY5Y cells were treated with EPA and/or BDNF. 
      The cell viability was determined by MTT assay. The expression of BDNF receptors, 
      tyrosine kinase receptor B (TrkB) and p75(NTR) were tested by RT-PCR and Western 
      blotting. RESULTS: In undifferentiated SH-SY5Y cells, either EPA or BDNF, or both 
      did not affect the cell viability. In RA-BDNF differentiated SH-SY5Y cells, 
      treatment with different doses of EPA (0.01, 0.1, 1.0, 10.0 microM) and BDNF (1 
      ng/ml) for 24 hours significantly increased the cell viability, while EPA or BDNF 
      alone showed no effect. More importantly, RT-PCR and Western blotting results 
      revealed that 24 hours treatment with EPA (0.01, 0.1, 1.0 microM) significantly 
      increased the full-length TrkB (TrkB(TK+)), but not truncated TrkB (TrkB(TK-)) 
      expression in these cells. An increase in p75(NTR) expression was also observed 
      with 10.0 microM EPA treatment. Finally, co-incubation with either 100 nM 
      staurosporine, a protein kinase inhibitor, or 500 nM K252a, a tyrosine kinase 
      inhibitor completely abolished the EPA-induced increase in cell viability. 
      CONCLUSIONS: Our results indicate that EPA exerts beneficial effects on cell 
      survival through modulating neurotrophin receptor expression.
FAU - Kou, Wei
AU  - Kou W
AD  - Dept. of Biomedical Sciences, AVC, University of Prince Edward Island, 
      Charlottetown (PE), Canada.
FAU - Luchtman, Dirk
AU  - Luchtman D
FAU - Song, Cai
AU  - Song C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080323
PL  - Germany
TA  - Eur J Nutr
JT  - European journal of nutrition
JID - 100888704
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 5688UTC01R (Tretinoin)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/drug effects/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - *Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Eicosapentaenoic Acid
MH  - Fatty Acids, Unsaturated/*pharmacology
MH  - Humans
MH  - Receptor, trkB/drug effects/metabolism
MH  - Receptors, Nerve Growth Factor/*drug effects/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tretinoin/metabolism
EDAT- 2008/03/25 09:00
MHDA- 2008/05/28 09:00
CRDT- 2008/03/25 09:00
PHST- 2007/06/13 00:00 [received]
PHST- 2008/03/05 00:00 [accepted]
PHST- 2008/03/25 09:00 [pubmed]
PHST- 2008/05/28 09:00 [medline]
PHST- 2008/03/25 09:00 [entrez]
AID - 10.1007/s00394-008-0703-1 [doi]
PST - ppublish
SO  - Eur J Nutr. 2008 Mar;47(2):104-13. doi: 10.1007/s00394-008-0703-1. Epub 2008 Mar 
      23.