PMID- 18361733
OWN - NLM
STAT- MEDLINE
DCOM- 20080806
LR  - 20091119
IS  - 1096-620X (Print)
IS  - 1096-620X (Linking)
VI  - 11
IP  - 1
DP  - 2008 Mar
TI  - Inhibitory effect of honeybee-collected pollen on mast cell degranulation in vivo 
      and in vitro.
PG  - 14-20
LID - 10.1089/jmf.2006.163 [doi]
AB  - Bee-collected pollen (bee pollen [BP]) has been used as a folk medicine for 
      centuries against various diseases, including allergy. There is no study 
      elucidating how BP exerts such an anti-allergic effect. Since mast cells play a 
      central role in the pathogenesis of various allergic diseases, we investigated 
      the effect of BP on mast cell activation elicited by the Fc immunoglobulin E 
      (IgE) receptor (Fc epsilon RI)-mediated pathways. The in vivo effect of orally 
      administered BP on cutaneous mast cell activation was examined by passive 
      cutaneous anaphylaxis reaction. In vitro mast cell degranulation and IgE binding 
      to mast cells and the status of protein tyrosine phosphorylation were examined 
      using bone marrow-derived mast cells. Daily oral administration of BP to mice 
      significantly reduced the cutaneous mast cell activation elicited by IgE and 
      specific antigens. BP also reduced in vitro mast cell degranulation and tumor 
      necrosis factor-alpha production by inhibiting IgE binding to Fc epsilon RI on 
      mast cells. The inhibitory effect of BP on mast cell degranulation by preventing 
      IgE binding was confirmed by the reduced levels of protein tyrosine 
      phosphorylation, which occurred as downstream events in activated mast cells via 
      Fc epsilon RI. These results first revealed that the anti-allergic action of BP 
      was exerted by inhibiting the Fc epsilon RI-mediated activation of mast cells, 
      which plays important roles, not only in the early phase, but also in the late 
      phase of allergic reactions.
FAU - Ishikawa, Yasuko
AU  - Ishikawa Y
AD  - Atopy (Allergy) Research Center, Juntendo University School of Medicine, Tokyo, 
      Japan.
FAU - Tokura, Tomoko
AU  - Tokura T
FAU - Nakano, Nobuhiro
AU  - Nakano N
FAU - Hara, Mutsuko
AU  - Hara M
FAU - Niyonsaba, Francois
AU  - Niyonsaba F
FAU - Ushio, Hiroko
AU  - Ushio H
FAU - Yamamoto, Yuji
AU  - Yamamoto Y
FAU - Tadokoro, Tadahiro
AU  - Tadokoro T
FAU - Okumura, Ko
AU  - Okumura K
FAU - Ogawa, Hideoki
AU  - Ogawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Cytokines)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - *Bees
MH  - Bone Marrow Cells
MH  - Cell Degranulation/*drug effects
MH  - Cytokines/biosynthesis
MH  - Immunoglobulin E/drug effects/immunology
MH  - Mast Cells/*drug effects/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Passive Cutaneous Anaphylaxis/drug effects
MH  - Phosphorylation/drug effects
MH  - Pollen/*chemistry
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Receptors, IgE/physiology
EDAT- 2008/03/26 09:00
MHDA- 2008/08/07 09:00
CRDT- 2008/03/26 09:00
PHST- 2008/03/26 09:00 [pubmed]
PHST- 2008/08/07 09:00 [medline]
PHST- 2008/03/26 09:00 [entrez]
AID - 10.1089/jmf.2006.163 [doi]
PST - ppublish
SO  - J Med Food. 2008 Mar;11(1):14-20. doi: 10.1089/jmf.2006.163.