PMID- 18362126
OWN - NLM
STAT- MEDLINE
DCOM- 20080703
LR  - 20211020
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 76
IP  - 6
DP  - 2008 Jun
TI  - Characterization of murine dendritic cell line JAWS II and primary bone 
      marrow-derived dendritic cells in Chlamydia muridarum antigen presentation and 
      induction of protective immunity.
PG  - 2392-401
LID - 10.1128/IAI.01584-07 [doi]
AB  - Dendritic cells (DCs) appear to orchestrate much of the immunobiology of 
      Chlamydia infection, but most studies of Chlamydia-DC interaction have been 
      limited by the availability and heterogeneity of primary bone marrow-derived DCs 
      (BMDCs). We therefore evaluated the immunobiology of Chlamydia muridarum 
      infection in an immortal DC line termed JAWS II derived from BMDCs of a C57BL/6 
      p53-knockout mouse. JAWS II cells were permissive to the developmental cycle of 
      Chlamydia. Infection-induced cell death was 50 to 80% less in JAWS II cells than 
      in BMDCs. Chlamydia infected JAWS II cells and yielded infectious progeny 10-fold 
      greater than that with primary BMDCs. JAWS II cells showed an expression pattern 
      of cell activation markers and cytokine secretion following Chlamydia infection 
      similar to that of primary BMDCs by up-regulating the expression of CD86, CD40, 
      and major histocompatibility complex class II and secreting significant amounts 
      of interleukin-12 (IL-12) but not IL-10. JAWS II cells pulsed with Chlamydia 
      stimulated immune CD4(+) T cells to secrete gamma interferon. Adoptive transfer 
      of ex vivo Chlamydia-pulsed JAWS II cells conferred levels of immunity on C57BL/6 
      mice similar to those conferred by primary BMDCs. Taken together, the data show 
      that JAWS II cells exhibit immunobiological characteristics and functions similar 
      to those of primary BMDCs in terms of Chlamydia antigen presentation in vitro and 
      antigen delivery in vivo. We conclude that the JAWS II cell line can substitute 
      for primary BMDCs in Chlamydia immunobiological studies.
FAU - Jiang, Xiaozhou
AU  - Jiang X
AD  - University of British Columbia Centre for Disease Control, 655 West 12th Avenue, 
      Vancouver, BC V5Z 4R4, Canada.
FAU - Shen, Caixia
AU  - Shen C
FAU - Rey-Ladino, Jose
AU  - Rey-Ladino J
FAU - Yu, Hong
AU  - Yu H
FAU - Brunham, Robert C
AU  - Brunham RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080324
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antibodies, Bacterial/biosynthesis
MH  - Antigen Presentation/*immunology
MH  - Antigens, Bacterial/*immunology
MH  - Biomarkers
MH  - Bone Marrow Cells/*cytology/immunology/microbiology
MH  - CD4-Positive T-Lymphocytes/metabolism
MH  - Cell Line
MH  - Chlamydia muridarum/*immunology/physiology
MH  - Dendritic Cells/*cytology/immunology/microbiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Specific Pathogen-Free Organisms
MH  - Up-Regulation
PMC - PMC2423070
EDAT- 2008/03/26 09:00
MHDA- 2008/07/04 09:00
PMCR- 2008/10/01
CRDT- 2008/03/26 09:00
PHST- 2008/03/26 09:00 [pubmed]
PHST- 2008/07/04 09:00 [medline]
PHST- 2008/03/26 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - IAI.01584-07 [pii]
AID - 1584-07 [pii]
AID - 10.1128/IAI.01584-07 [doi]
PST - ppublish
SO  - Infect Immun. 2008 Jun;76(6):2392-401. doi: 10.1128/IAI.01584-07. Epub 2008 Mar 
      24.