PMID- 18362183
OWN - NLM
STAT- MEDLINE
DCOM- 20080429
LR  - 20211020
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 180
IP  - 6
DP  - 2008 Mar 24
TI  - WASP family members and formin proteins coordinate regulation of cell protrusions 
      in carcinoma cells.
PG  - 1245-60
LID - 10.1083/jcb.200708123 [doi]
AB  - We examined the role of the actin nucleation promoters neural Wiskott-Aldrich 
      syndrome protein (N-WASP) and WAVE2 in cell protrusion in response to epidermal 
      growth factor (EGF), a key regulator in carcinoma cell invasion. We found that 
      WAVE2 knockdown (KD) suppresses lamellipod formation and increases filopod 
      formation, whereas N-WASP KD has no effect. However, simultaneous KD of both 
      proteins results in the formation of large jagged protrusions with lamellar 
      properties and increased filopod formation. This suggests that another actin 
      nucleation activity is at work in carcinoma cells in response to EGF. A mammalian 
      Diaphanous-related formin, mDia1, localizes at the jagged protrusions in double 
      KD cells. Constitutively active mDia1 recapitulated the phenotype, whereas 
      inhibition of mDia1 blocked the formation of these protrusions. Increased RhoA 
      activity, which stimulates mDia1 nucleation, was observed in the N-WASP/WAVE2 KD 
      cells and was shown to be required for the N-WASP/WAVE2 KD phenotype. These data 
      show that coordinate regulation between the WASP family and mDia proteins 
      controls the balance between lamellar and lamellipodial protrusion activity.
FAU - Sarmiento, Corina
AU  - Sarmiento C
AD  - Department of Anatomy and Structural Biology, Albert Einstein College of 
      Medicine, Yeshiva University, Bronx, NY 10461, USA. csarmien@aecom.yu.edu
FAU - Wang, Weigang
AU  - Wang W
FAU - Dovas, Athanassios
AU  - Dovas A
FAU - Yamaguchi, Hideki
AU  - Yamaguchi H
FAU - Sidani, Mazen
AU  - Sidani M
FAU - El-Sibai, Mirvat
AU  - El-Sibai M
FAU - Desmarais, Vera
AU  - Desmarais V
FAU - Holman, Holly A
AU  - Holman HA
FAU - Kitchen, Susan
AU  - Kitchen S
FAU - Backer, Jonathan M
AU  - Backer JM
FAU - Alberts, Art
AU  - Alberts A
FAU - Condeelis, John
AU  - Condeelis J
LA  - eng
GR  - R01 GM038511/GM/NIGMS NIH HHS/United States
GR  - GM38511/GM/NIGMS NIH HHS/United States
GR  - CA10023/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Carrier Proteins)
RN  - 0 (Diap1 protein, mouse)
RN  - 0 (Formins)
RN  - 0 (Wasl protein, rat)
RN  - 0 (Wiskott-Aldrich Syndrome Protein Family)
RN  - 0 (Wiskott-Aldrich Syndrome Protein, Neuronal)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 1.6.2.2 (Cyb5R3 protein, rat)
RN  - EC 1.6.2.2 (Cytochrome-B(5) Reductase)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Animals
MH  - Carcinoma/*metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects/*physiology
MH  - Cell Surface Extensions/drug effects/*metabolism/ultrastructure
MH  - Cytochrome-B(5) Reductase/genetics/*metabolism
MH  - Down-Regulation/physiology
MH  - Epidermal Growth Factor/metabolism/pharmacology
MH  - Formins
MH  - Neoplasm Invasiveness/physiopathology
MH  - Neoplasms/*metabolism
MH  - Pseudopodia/drug effects/metabolism/ultrastructure
MH  - Rats
MH  - Wiskott-Aldrich Syndrome Protein Family/genetics/*metabolism
MH  - Wiskott-Aldrich Syndrome Protein, Neuronal/genetics/*metabolism
MH  - rhoA GTP-Binding Protein/metabolism
PMC - PMC2290849
EDAT- 2008/03/26 09:00
MHDA- 2008/04/30 09:00
PMCR- 2008/09/24
CRDT- 2008/03/26 09:00
PHST- 2008/03/26 09:00 [pubmed]
PHST- 2008/04/30 09:00 [medline]
PHST- 2008/03/26 09:00 [entrez]
PHST- 2008/09/24 00:00 [pmc-release]
AID - jcb.200708123 [pii]
AID - 200708123 [pii]
AID - 10.1083/jcb.200708123 [doi]
PST - ppublish
SO  - J Cell Biol. 2008 Mar 24;180(6):1245-60. doi: 10.1083/jcb.200708123.