PMID- 18362472
OWN - NLM
STAT- MEDLINE
DCOM- 20080729
LR  - 20240109
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 146
IP  - 4
DP  - 2008
TI  - Characterization of IgE binding to lupin, peanut and almond with sera from 
      lupin-allergic patients.
PG  - 267-76
LID - 10.1159/000121461 [doi]
AB  - BACKGROUND: The increasing number of applications of sweet lupins in food is 
      paralleled by an increase in immunoglobulin E (IgE)-mediated allergic reactions 
      to lupin proteins. In particular, lupin allergy seems to appear in patients with 
      an existing peanut allergy. In the present study, IgE-binding studies towards 
      fractionated lupin seed proteins, and peanut and almond proteins were performed 
      using sera from patients with confirmed lupin allergy. METHODS: Immunoblotting 
      and indirect ELISA were performed to investigate IgE binding to protein extracts. 
      ELISA inhibition experiments were performed to investigate the presence of 
      cross-reactive allergens in the protein extracts. RESULTS: Immunoblotting and 
      ELISA experiments demonstrated IgE binding to all lupin conglutins (alpha, beta, 
      gamma and delta) as well as to peanut and almond proteins, with a unique 
      IgE-binding profile for each patient. High IgE binding to alpha-conglutin was 
      observed and IgE from the majority of patients similarly recognized two proteins 
      within the alpha-conglutin-containing fraction, 40 and 43 kDa in size. Inhibition 
      ELISA experiments showed that preincubation of sera with lupin conglutins, peanut 
      and almond resulted in decreased IgE binding to lupin flour. CONCLUSIONS: 
      Overall, these results indicate that alpha-, beta-, gamma- and delta-conglutins 
      are candidate allergens in lupin and suggest a particularly strong allergenicity 
      of alpha-conglutins. Furthermore, the results indicate the presence of 
      cross-reactive allergens in lupin, peanut and almond.
CI  - Copyright 2008 S. Karger AG, Basel.
FAU - Holden, Lise
AU  - Holden L
AD  - National Veterinary Institute, Oslo, Norway.
FAU - Sletten, Gaynour B G
AU  - Sletten GB
FAU - Lindvik, Helene
AU  - Lindvik H
FAU - Faeste, Christiane K
AU  - Faeste CK
FAU - Dooper, Maaike M B W
AU  - Dooper MM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080321
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Plant Proteins)
RN  - 0 (Seed Storage Proteins)
RN  - 1391-07-7 (conglutin protein, Lupinus angustifolius)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Arachis/*immunology
MH  - Child
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Food Hypersensitivity/blood/*immunology
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/blood/*immunology
MH  - Lupinus/*immunology
MH  - Male
MH  - Plant Proteins/*immunology
MH  - Prunus/*immunology
MH  - Seed Storage Proteins
EDAT- 2008/03/26 09:00
MHDA- 2008/07/30 09:00
CRDT- 2008/03/26 09:00
PHST- 2007/07/10 00:00 [received]
PHST- 2007/11/20 00:00 [accepted]
PHST- 2008/03/26 09:00 [pubmed]
PHST- 2008/07/30 09:00 [medline]
PHST- 2008/03/26 09:00 [entrez]
AID - 000121461 [pii]
AID - 10.1159/000121461 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2008;146(4):267-76. doi: 10.1159/000121461. Epub 2008 
      Mar 21.