PMID- 18362665
OWN - NLM
STAT- MEDLINE
DCOM- 20080508
LR  - 20131121
IS  - 0277-3740 (Print)
IS  - 0277-3740 (Linking)
VI  - 27
IP  - 3
DP  - 2008 Apr
TI  - Corneal, limbal, and conjunctival epithelium of bovine eyes imaged in vitro by 
      using a confocal laser scanning microscope.
PG  - 344-8
LID - 10.1097/ICO.0b013e31815caa7f [doi]
AB  - PURPOSE: To provide a description of the distribution and cell morphology, by 
      using fluorescent markers and confocal laser scanning microscopy, of the corneal, 
      limbal, and conjunctival epithelium of bovine eyes in vitro. METHODS: Fresh 
      enucleated bovine eyes were dissected within 2 hours postmortem. Central cornea, 
      limbus, and bulbar conjunctiva were imaged with confocal microscopy after 
      staining with acridine orange (AO) or calcein-acetoxymethyl and ethidium 
      homodimer-1. Epithelial thickness, cell density, cell lamination, and cell 
      morphology were evaluated at these 3 locations. RESULTS: Corneal epithelium was 
      the thickest, and the conjunctival epithelium was the thinnest. The cell 
      morphology was similar to that found in previous histologic studies, and the cell 
      density gradually decreased from the basal to superficial layers. Nuclear AO 
      staining particles increased from basal to superficial cells. Limbal superficial 
      epithelial cells showed less AO staining than corneal and conjunctival 
      superficial cells. CONCLUSIONS: Confocal results of the corneal central, limbal, 
      and conjunctival morphology are similar to those found in traditional microscopic 
      observations. Bovine central corneal epithelium is thicker than limbal 
      epithelium. However, the nuclear AO staining pattern of unfixed ocular surface 
      epithelium of bovine eyes in vitro might represent the cell differentiation 
      status. With the aid of the fluorescence dye, confocal laser scanning microscopy 
      can provide unique morphometric information about corneal, limbal, and 
      conjunctival epithelial cells.
FAU - Feng, Yunwei
AU  - Feng Y
AD  - Centre for Contact Lens Research, University of Waterloo, Waterloo, Ontario, 
      Canada.
FAU - Bantseev, Vladimir
AU  - Bantseev V
FAU - Simpson, Trefford L
AU  - Simpson TL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 148504-34-1 (calcein AM)
RN  - 61926-22-5 (ethidium homodimer)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Animals
MH  - Cattle
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Conjunctiva/*cytology
MH  - Epithelial Cells/*cytology
MH  - Epithelium, Corneal/*cytology
MH  - Ethidium/analogs & derivatives
MH  - Fluoresceins
MH  - Fluorescent Dyes
MH  - Limbus Corneae/*cytology
MH  - *Microscopy, Confocal
EDAT- 2008/03/26 09:00
MHDA- 2008/05/09 09:00
CRDT- 2008/03/26 09:00
PHST- 2008/03/26 09:00 [pubmed]
PHST- 2008/05/09 09:00 [medline]
PHST- 2008/03/26 09:00 [entrez]
AID - 00003226-200804000-00018 [pii]
AID - 10.1097/ICO.0b013e31815caa7f [doi]
PST - ppublish
SO  - Cornea. 2008 Apr;27(3):344-8. doi: 10.1097/ICO.0b013e31815caa7f.