PMID- 18363573
OWN - NLM
STAT- MEDLINE
DCOM- 20080801
LR  - 20080501
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 21
IP  - 6
DP  - 2008 Jun
TI  - Administration of interleukin-1 receptor antagonist ameliorates renal 
      ischemia-reperfusion injury.
PG  - 572-80
LID - 10.1111/j.1432-2277.2008.00651.x [doi]
AB  - Interleukin (IL)-1 is a major contributor to inflammation and apoptosis during 
      ischemia/reperfusion (I/R) injury. Its deleterious effects are primarily mediated 
      by the activation of nuclear factor-kappaB (NF-kappaB). Receptor-binding and 
      signaling of IL-1 can be blocked by the IL-1 receptor antagonist (IL-1ra). The 
      aim of our study was to characterize effects and mechanisms of IL-1ra 
      administration on inflammation, apoptosis, and infiltration in renal I/R injury. 
      Renal ischemia was induced in Lewis rats by clamping of the left renal artery for 
      45 min. Kidneys were removed for histological and molecular analysis 24 h or 5 
      days after reperfusion. IL-1ra ameliorated I/R induced renal injury and 
      inflammation. Furthermore, the number of apoptotic tubular cells was lower in 
      IL-1ra-treated animals 24 h after ischemia, which was paralleled by a Bax/Bcl-2 
      mRNA ratio towards anti-apoptotic effects. IL-1ra reduced the expression of 
      monocyte chemoattractant protein-1 (MCP-1) mRNA at 24 h and 5 days and that of 
      intracellular adhesion molecule-1 (ICAM-1) expression at 24 h in the ischemic 
      reperfused kidneys. Our results indicate that IL-1ra treatment ameliorates renal 
      I/R injury and this protective effect might be mediated by reduced induction of 
      NF-kappaB mediated MCP-1, ICAM-1, and a decreased ratio between Bax and Bcl-2 
      mRNA expression.
FAU - Rusai, Krisztina
AU  - Rusai K
AD  - First Department of Pediatrics, Semmelweis University, Budapest, Hungary.
FAU - Huang, Hai
AU  - Huang H
FAU - Sayed, Nora
AU  - Sayed N
FAU - Strobl, Matthias
AU  - Strobl M
FAU - Roos, Marcel
AU  - Roos M
FAU - Schmaderer, Christoph
AU  - Schmaderer C
FAU - Heemann, Uwe
AU  - Heemann U
FAU - Lutz, Jens
AU  - Lutz J
LA  - eng
PT  - Journal Article
DEP - 20080318
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (Bax protein, rat)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin 1 Receptor Antagonist Protein)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Base Sequence
MH  - Chemokine CCL2/genetics
MH  - DNA Primers/genetics
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Interleukin 1 Receptor Antagonist Protein/*administration & dosage
MH  - Kidney/blood supply/*drug effects/*injuries/pathology
MH  - Leukocytes/drug effects/pathology
MH  - Male
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Recombinant Proteins/administration & dosage
MH  - Reperfusion Injury/genetics/pathology/*prevention & control
MH  - bcl-2-Associated X Protein/genetics
EDAT- 2008/03/28 09:00
MHDA- 2008/08/02 09:00
CRDT- 2008/03/28 09:00
PHST- 2008/03/28 09:00 [pubmed]
PHST- 2008/08/02 09:00 [medline]
PHST- 2008/03/28 09:00 [entrez]
AID - TRI651 [pii]
AID - 10.1111/j.1432-2277.2008.00651.x [doi]
PST - ppublish
SO  - Transpl Int. 2008 Jun;21(6):572-80. doi: 10.1111/j.1432-2277.2008.00651.x. Epub 
      2008 Mar 18.