PMID- 18364357
OWN - NLM
STAT- MEDLINE
DCOM- 20080718
LR  - 20240322
IS  - 0021-9258 (Print)
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Linking)
VI  - 283
IP  - 21
DP  - 2008 May 23
TI  - Monocyte chemotactic protein (MCP)-1 promotes angiogenesis via a novel 
      transcription factor, MCP-1-induced protein (MCPIP).
PG  - 14542-51
LID - 10.1074/jbc.M802139200 [doi]
AB  - Monocyte chemotactic protein-1 (MCP-1) has been recognized as an angiogenic 
      chemokine. The molecular mechanism of MCP-1-mediated angiogenesis remains 
      unknown. We recently identified a novel transcription factor, designated 
      MCP-1-induced protein (MCPIP), in human monocytes after treatment with MCP-1. We 
      investigated whether MCP-1-induced angiogenesis is mediated via MCPIP. Treatment 
      of human umbilical vein endothelial cells (HUVECs) with MCP-1 induced expression 
      of MCPIP and capillary-like tube formation. Knockdown of MCPIP by small 
      interfering RNA (siRNA) suppressed MCP-1-induced angiogenesis-related gene VEGF 
      and HIF-1alpha expression as well as tube formation. Transfection of HUVECs with 
      an MCPIP expression vector induced angiogenesis-related genes and tube formation. 
      Chromatin immunoprecipitation analysis revealed that cadherin (cdh) 12 and cdh19 
      are in vivo targets of MCPIP. Transfection of HUVECs with MCPIP expression vector 
      activated the expression of cdh12 and cdh19 genes. Knockdown of cdh12 or cdh19 
      expression markedly inhibited MCPIP-induced capillary-like tube formation. 
      Moreover, knockdown of MCPIP also significantly suppressed MCP-1-induced cdh12 
      and cdh19 gene expression. Our data strongly suggest that MCP-1-induced 
      angiogenesis is mediated via MCPIP, at least in part through transcriptional 
      activation of cdh12 and cdh19.
FAU - Niu, Jianli
AU  - Niu J
AD  - Burnett School of Biomedical Sciences, College of Medicine, University of Central 
      Florida, Orlando, FL 32816, USA.
FAU - Azfer, Asim
AU  - Azfer A
FAU - Zhelyabovska, Olga
AU  - Zhelyabovska O
FAU - Fatma, Sumbul
AU  - Fatma S
FAU - Kolattukudy, Pappachan E
AU  - Kolattukudy PE
LA  - eng
GR  - HL 69458/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080324
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cadherins)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Transcription Factors)
RN  - EC 3.1.- (Ribonucleases)
RN  - EC 3.1.- (ZC3H12A protein, human)
SB  - IM
MH  - Cadherins/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Endothelial Cells/metabolism
MH  - Gene Expression Profiling
MH  - Humans
MH  - *Neovascularization, Physiologic
MH  - RNA, Small Interfering/genetics
MH  - Ribonucleases
MH  - Transcription Factors/genetics/*metabolism
MH  - Up-Regulation
PMC - PMC2386911
EDAT- 2008/03/28 09:00
MHDA- 2008/07/19 09:00
PMCR- 2009/05/23
CRDT- 2008/03/28 09:00
PHST- 2008/03/28 09:00 [pubmed]
PHST- 2008/07/19 09:00 [medline]
PHST- 2008/03/28 09:00 [entrez]
PHST- 2009/05/23 00:00 [pmc-release]
AID - S0021-9258(20)54322-6 [pii]
AID - 14542 [pii]
AID - 10.1074/jbc.M802139200 [doi]
PST - ppublish
SO  - J Biol Chem. 2008 May 23;283(21):14542-51. doi: 10.1074/jbc.M802139200. Epub 2008 
      Mar 24.