PMID- 1836684
OWN - NLM
STAT- MEDLINE
DCOM- 19920127
LR  - 20190727
IS  - 0040-8727 (Print)
IS  - 0040-8727 (Linking)
VI  - 164
IP  - 3
DP  - 1991 Jul
TI  - Pharmacokinetic analysis of the monoclonal antibody A7-neocarzinostatin conjugate 
      administered to nude mice.
PG  - 203-11
AB  - The pharmacokinetics of a disulfide linked conjugate of a murine monoclonal 
      antibody A7 with neocarzinostatin (A7-NCS) was studied following its intravenous 
      administration to nude mice. Disappearance of the conjugate from the circulation 
      was biphasic: an early rapid phase was followed by a much slower phase. The 
      conjugate was removed from the blood circulation with a half-life of 12 hr, 
      showing nearly the same kinetics as the free antibody. Sodium dodecyl 
      sulfate-polyacrylamide gel electrophoresis analysis showed that the disulfide 
      linkage in A7-NCS was stable at least for 48 hr after administration of the 
      conjugate to nude mice. The conjugate concentration in a human colon cancer 
      SW1116 derived tumor reached maximum at 24 hr after injection and remained high 
      for an additional 24 hr. The passive hemagglutination inhibition assay revealed 
      that NCS in the conjugated form can be efficiently delivered to the target 
      tissue. The present report indicates that A7-NCS was sufficiently stable in 
      circulation to reach the target tumor without releasing NCS.
FAU - Kitamura, K
AU  - Kitamura K
AD  - First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
FAU - Takahashi, T
AU  - Takahashi T
FAU - Noguchi, A
AU  - Noguchi A
FAU - Tsurumi, H
AU  - Tsurumi H
FAU - Takashina, K
AU  - Takashina K
FAU - Okuzumi, J
AU  - Okuzumi J
FAU - Yamaguchi, T
AU  - Yamaguchi T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Drug Carriers)
RN  - 0 (Iodine Radioisotopes)
RN  - 9014-02-2 (Zinostatin)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/administration & dosage/*metabolism
MH  - Colonic Neoplasms/metabolism
MH  - Drug Carriers
MH  - Drug Stability
MH  - Half-Life
MH  - Humans
MH  - Injections, Intravenous
MH  - Iodine Radioisotopes/pharmacokinetics
MH  - Mice
MH  - Mice, Nude/metabolism
MH  - Neoplasm Transplantation
MH  - Neoplasms, Experimental/diagnostic imaging
MH  - Radioimmunodetection
MH  - Transplantation, Heterologous
MH  - Zinostatin/administration & dosage/*pharmacokinetics
EDAT- 1991/07/01 00:00
MHDA- 1991/07/01 00:01
CRDT- 1991/07/01 00:00
PHST- 1991/07/01 00:00 [pubmed]
PHST- 1991/07/01 00:01 [medline]
PHST- 1991/07/01 00:00 [entrez]
AID - 10.1620/tjem.164.203 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 1991 Jul;164(3):203-11. doi: 10.1620/tjem.164.203.