PMID- 18369508
OWN - NLM
STAT- MEDLINE
DCOM- 20080702
LR  - 20151119
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 8
IP  - 4
DP  - 2008 Apr
TI  - A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and 
      detection capabilities.
PG  - 542-9
LID - 10.1039/b715569g [doi]
AB  - This paper presents a new polymer lab-on-a-chip for magnetic bead-based 
      immunoassay with fully on-chip sampling and detection capabilities, which 
      provides a smart platform of magnetic immunoassay-based lab-on-a-chip for 
      point-of-care testing (POCT) toward biochemical hazardous agent detection, food 
      inspection or clinical diagnostics. In this new approach, the polymer 
      lab-on-a-chip for magnetic bead-based immunoassay consists of a magnetic 
      bead-based separator, an interdigitated array (IDA) micro electrode, and a 
      microfluidic system, which are fully incorporated into a lab-on-a-chip on cyclic 
      olefin copolymer (COC). Since the polymer lab-on-a-chip was realized using low 
      cost, high throughput polymer microfabrication techniques such as micro injection 
      molding and hot embossing method, a disposable polymer lab-on-a-chip for the 
      magnetic bead-based immunoassay can be successfully realized in a disposable 
      platform. With this newly developed polymer lab-on-a-chip, an enzyme-labelled 
      electrochemical immunoassay (ECIA) was performed using magnetic beads as the 
      mobile solid support, and the final enzyme product produced from the ECIA was 
      measured using chronoamperometry. A sampling and detection of as low as 16.4 ng 
      mL(-1) of mouse IgG has been successfully performed in 35 min for the entire 
      procedure.
FAU - Do, Jaephil
AU  - Do J
AD  - Microsystems and BioMEMS Laboratory, Center for BioMEMS and Nanobiosciences, 
      Department of Electrical and Computer Engineering and Computer Science, 
      University of Cincinnati, Cincinnati, OH 45221, USA. dohj@ececs.uc.edu
FAU - Ahn, Chong H
AU  - Ahn CH
LA  - eng
GR  - NIH-P021-L684/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20080228
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Immunoglobulin G)
RN  - 0 (Polymers)
SB  - IM
MH  - Animals
MH  - Biosensing Techniques/economics/instrumentation/*methods
MH  - Electrochemistry
MH  - Immunoassay/economics/instrumentation/*methods
MH  - Immunoglobulin G/*analysis/metabolism
MH  - Lab-On-A-Chip Devices
MH  - *Magnetics
MH  - Mice
MH  - Microchip Analytical Procedures/economics/*methods
MH  - Polymers/*chemistry
MH  - Sensitivity and Specificity
MH  - Time Factors
EDAT- 2008/03/29 09:00
MHDA- 2008/07/03 09:00
CRDT- 2008/03/29 09:00
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2008/07/03 09:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - 10.1039/b715569g [doi]
PST - ppublish
SO  - Lab Chip. 2008 Apr;8(4):542-9. doi: 10.1039/b715569g. Epub 2008 Feb 28.