PMID- 18369922
OWN - NLM
STAT- MEDLINE
DCOM- 20080515
LR  - 20131121
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 447
DP  - 2008
TI  - Dendritic cells in chronic in vivo ethanol exposure models.
PG  - 213-33
LID - 10.1007/978-1-59745-242-7_16 [doi]
AB  - Dendritic cells (DCs) play a key role in the initiation of effective immune 
      responses against infectious agents because they are unique in their ability to 
      provide antigen-specific activation of naive T cells. To do this, they must 
      acquire antigen and migrate to spleen or lymph node to present the antigen to T 
      cells in association with costimulatory molecules and cytokines. Murine models of 
      chronic EtOH exposure have been developed for dissecting the mechanisms by which 
      EtOH alters immune cell functions. This chapter details methods for assessing DC 
      functions in such models. Methods are presented for 1) the identification and 
      isolation of various DC subsets from spleen, epidermis, and lung, 2) measurement 
      of LC migration out of epidermis and DC migration into peripheral and 
      peribronchial lymph nodes, and 3) measurement of alloantigen presentation in 
      vitro as well as transgenic T-cell activation in vitro and in vivo.
FAU - Legge, Kevin L
AU  - Legge KL
AD  - Department of Pathology, University of Iowa Carver College of Medicine, Iowa 
      City, IA, USA.
FAU - Schlueter, Annette J
AU  - Schlueter AJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Alcohol Drinking/*immunology
MH  - Alcoholism/*immunology
MH  - Animals
MH  - Antigen Presentation
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Culture Techniques
MH  - Cell Movement
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Ethanol/*administration & dosage
MH  - Langerhans Cells/*immunology
MH  - Lymphocyte Activation
MH  - Mice
EDAT- 2008/03/29 09:00
MHDA- 2008/05/16 09:00
CRDT- 2008/03/29 09:00
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2008/05/16 09:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - 10.1007/978-1-59745-242-7_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;447:213-33. doi: 10.1007/978-1-59745-242-7_16.