PMID- 18369941
OWN - NLM
STAT- MEDLINE
DCOM- 20080515
LR  - 20161124
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 440
DP  - 2008
TI  - Identification and characterization of interacting partners of Rab GTPases by 
      yeast two-hybrid analyses.
PG  - 111-25
LID - 10.1007/978-1-59745-178-9_9 [doi]
AB  - Much of our knowledge of the mechanisms governing vesicular transport has come 
      from the combination of genetic and biochemical approaches that have identified 
      Ypt/Rab guanosine 5'-triphosphatases (GTPases) as key components of transport 
      processes in both yeast and mammalian cells. More recently, research has focused 
      on establishing the complex protein-protein interactions necessary for the 
      regulation of vesicular transport by a variety of methods, including the yeast 
      two-hybrid interaction assay. A central component of the signaling pathway 
      regulated by Ypt/Rab proteins is the GTPase cycle, in which the proteins cycle 
      between an active guanosine 5'-triphosphate (GTP)-bound form and an inactive 
      guanosine 5'-diphosphate (GDP)-bound form. Alterations in the conformation of the 
      Ypt/Rab proteins when either GTP or GDP is bound specify the interaction of 
      effector proteins and influence membrane binding. Our work has focused on 
      identifying interacting partners for the GTPases Rab1 and Rab6 and their 
      isoforms, which regulate transport steps between the endoplasmic reticulum and 
      Golgi in mammalian cells. We have employed both active (GTP-bound) and inactive 
      (GDP-bound) Rab1 and Rab6 mutants to identify potential new interacting proteins 
      using the yeast two-hybrid system and have verified these interactions using 
      alternative methods.
FAU - Kail, Mark
AU  - Kail M
AD  - Department of Experimental Tumor Biology, University of Muenster, Muenster, 
      Germany.
FAU - Barnekow, Angelika
AU  - Barnekow A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fungal Proteins)
RN  - 0 (Rab6 protein)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rab1 GTP-Binding Proteins)
SB  - IM
MH  - Fungal Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Fungal
MH  - Guanosine Diphosphate/metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Mutation
MH  - Protein Binding
MH  - *Signal Transduction
MH  - Transport Vesicles/*enzymology
MH  - *Two-Hybrid System Techniques
MH  - Yeasts/*enzymology/genetics
MH  - rab GTP-Binding Proteins/genetics/*metabolism
MH  - rab1 GTP-Binding Proteins/genetics/*metabolism
EDAT- 2008/03/29 09:00
MHDA- 2008/05/16 09:00
CRDT- 2008/03/29 09:00
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2008/05/16 09:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - 10.1007/978-1-59745-178-9_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;440:111-25. doi: 10.1007/978-1-59745-178-9_9.