PMID- 18369950
OWN - NLM
STAT- MEDLINE
DCOM- 20080515
LR  - 20080328
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 440
DP  - 2008
TI  - Visualizing clathrin-mediated IgE receptor internalization by electron and atomic 
      force microscopy.
PG  - 235-45
LID - 10.1007/978-1-59745-178-9_18 [doi]
AB  - A significant step in the immunoglobulin E (IgE) receptor signaling pathway in 
      mast cell membranes is receptor internalization by clathrin-coated vesicles. 
      Visualization in native membrane sheets of the emerging clathrin lattice 
      structures containing the IgE receptor and associated signaling partners has been 
      accomplished with high-resolution transmission electron microscopy (TEM). More 
      recently, membrane sheets with labeled clathrin have also been characterized with 
      atomic force microscopy (AFM) in combination with fluorescence imaging. We 
      discuss here the procedure for creating fixed, native cell membrane sheets, 
      labeling with immunogold or fluorescent labels, and utilization for TEM or 
      AFM/fluorescence imaging of clathrin-mediated IgE internalization.
FAU - Burns, Alan R
AU  - Burns AR
AD  - Biomolecular Interfaces and Systems Department, Sandia National Laboratories, 
      Albuquerque, NM, USA.
FAU - Oliver, Janet M
AU  - Oliver JM
FAU - Pfeiffer, Janet R
AU  - Pfeiffer JR
FAU - Wilson, Bridget S
AU  - Wilson BS
LA  - eng
GR  - A1051575/PHS HHS/United States
GR  - CA119232/CA/NCI NIH HHS/United States
GR  - GM49814/GM/NIGMS NIH HHS/United States
GR  - P20 GM067594/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Clathrin-Coated Vesicles/*metabolism/ultrastructure
MH  - Coated Pits, Cell-Membrane/*metabolism/ultrastructure
MH  - *Endocytosis
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Immunohistochemistry
MH  - Mast Cells/*metabolism/ultrastructure
MH  - *Microscopy, Atomic Force
MH  - *Microscopy, Electron, Transmission
MH  - Protein Transport
MH  - Receptors, IgE/*metabolism
EDAT- 2008/03/29 09:00
MHDA- 2008/05/16 09:00
CRDT- 2008/03/29 09:00
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2008/05/16 09:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - 10.1007/978-1-59745-178-9_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;440:235-45. doi: 10.1007/978-1-59745-178-9_18.