PMID- 18370006
OWN - NLM
STAT- MEDLINE
DCOM- 20080603
LR  - 20111117
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 427
DP  - 2008
TI  - Detection of programmed cell death in plant embryos.
PG  - 173-9
LID - 10.1007/978-1-59745-273-1_14 [doi]
AB  - Programmed cell death (PCD) is an integral part of embryogenesis. In plant 
      embryos, PCD functions during terminal differentiation and elimination of the 
      temporary organ, suspensor, as well as during establishment of provascular 
      system. Embryo abortion is another example of embryonic PCD activated at 
      pathological situations and in polyembryonic seeds. Recent studies identified the 
      sequence of cytological events leading to cellular self-destruction in plant 
      embryos. As in most if not all the developmental cell deaths in plants, embryonic 
      PCD is hallmarked by autophagic degradation of the cytoplasm and nuclear 
      disassembly that includes breakdown of the nuclear envelope and DNA 
      fragmentation. The optimized setup of terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) allows the routine in situ 
      analysis of nuclear DNA fragmentation in plant embryos. This chapter provides 
      step-by-step procedure of how to process embryos for TUNEL and how to combine 
      TUNEL with immunolocalization of the protein of interest.
FAU - Filonova, Lada H
AU  - Filonova LH
AD  - Department of Wood Sciences, Swedish University of Agricultural Sciences, 
      Uppsala, Sweden.
FAU - Suarez, Maria F
AU  - Suarez MF
FAU - Bozhkov, Peter V
AU  - Bozhkov PV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Coloring Agents)
SB  - IM
MH  - Apoptosis
MH  - Cell Differentiation
MH  - Coloring Agents
MH  - In Situ Nick-End Labeling
MH  - Plant Cells
MH  - Plants/*embryology
MH  - Seeds/*cytology/*physiology
EDAT- 2008/03/29 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/03/29 09:00
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - 10.1007/978-1-59745-273-1_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;427:173-9. doi: 10.1007/978-1-59745-273-1_14.