PMID- 18371057
OWN - NLM
STAT- MEDLINE
DCOM- 20090917
LR  - 20090703
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 31
IP  - 4
DP  - 2009 Aug
TI  - Refinement and feasibility testing of a manual micro-method for protamine 
      titration.
PG  - 457-61
LID - 10.1111/j.1751-553X.2008.01055.x [doi]
AB  - Current clinical recommendations for unfractionated heparin (UFH) therapy suggest 
      target APTT ranges should reflect heparin concentrations of 0.2-0.4 IU/ml by 
      protamine titration or 0.35-0.7 IU/m by an anti-Xa assay. Historically, 
      performance of a manual protamine titration assay has been labour intensive and 
      required a large plasma sample. However, recent studies have described 
      difficulties with standardizing anti-Xa assays and demonstrated poor correlation 
      of anti-Xa assays in children. This study aimed to refine and test the 
      feasibility of a modified protamine titration assay using 100 microl of plasma. 
      The resultant method produced reliable and repeatable results in adult plasma 
      pools spiked with UFH. The feasibility of this method was proven by testing of in 
      vivo heparinised samples obtained from children. This protamine titration method 
      may offer an alternative to anti-Xa assays for clinical monitoring of children on 
      heparin therapy, and will enhance clinical studies investigating 
      paediatric-specific management of UFH therapy.
FAU - Newall, F
AU  - Newall F
AD  - Department of Pathology, The University of Melbourne, Melbourne, Australia. 
      fiona.newall@rch.org.au
FAU - Johnston, L
AU  - Johnston L
FAU - Ignjatovic, V
AU  - Ignjatovic V
FAU - Summerhayes, R
AU  - Summerhayes R
FAU - Monagle, P
AU  - Monagle P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080321
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Protamines)
RN  - 9005-49-6 (Heparin)
RN  - EC 3.4.21.6 (Factor Xa)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Factor Xa/analysis
MH  - Heparin/*blood
MH  - Humans
MH  - Infant
MH  - Protamines/*blood
MH  - Titrimetry/*methods
EDAT- 2008/03/29 09:00
MHDA- 2009/09/18 06:00
CRDT- 2008/03/29 09:00
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2009/09/18 06:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - CLH1055 [pii]
AID - 10.1111/j.1751-553X.2008.01055.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2009 Aug;31(4):457-61. doi: 10.1111/j.1751-553X.2008.01055.x. 
      Epub 2008 Mar 21.