PMID- 18371739 OWN - NLM STAT- MEDLINE DCOM- 20080826 LR - 20161124 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 74 IP - 4 DP - 2008 Jan 15 TI - Rapid aryl hydrocarbon receptor based polymerase chain reaction screening assay for polychlorinated dibenzo-p-dioxins and furans in soil and sediment. PG - 992-7 LID - 10.1016/j.talanta.2007.08.005 [doi] AB - A method for the estimation of polychlorinated dibenzo-p-dioxin and furan (PCCD/F) toxicity equivalent quotient (TEQ) of soil and sediment matrices is described. The method includes extraction, isolation of the PCDD/Fs from interfering compounds, such as polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs), and measurement of PCDD/F using the PROCEPT aryl hydrocarbon (AhR) receptor based polymerase chain reaction (PCR) assay. The values obtained using the PROCEPT assay correlate well with reference TEQ values generated from gas chromatography-high resolution mass spectrometry (GC-HRMS), with a linearity coefficient (R(2)) of 0.94. Applied in a screening mode at 50pg/g PCDD/F TEQ, the PROCEPT assay yielded five false positive results (2.6%) and no false negative results for 196 analyses of spiked soils and environmental samples obtained from US EPA Superfund sites. FAU - McAlister, Daniel R AU - McAlister DR AD - Eichrom Technologies, LLC 8205 S Cass Avenue, Suite 106, Darien, IL 60561, United States. dmcalister@pgrf.com FAU - Fern, Michael J AU - Fern MJ FAU - Allen, Randy L AU - Allen RL LA - eng PT - Journal Article DEP - 20070814 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R RN - 0 (Benzofurans) RN - 0 (Dibenzofurans, Polychlorinated) RN - 0 (Polychlorinated Dibenzodioxins) RN - 0 (Receptors, Aryl Hydrocarbon) RN - 0 (Soil Pollutants) SB - IM MH - Benzofurans/*analysis MH - Dibenzofurans, Polychlorinated MH - Polychlorinated Dibenzodioxins/*analogs & derivatives/analysis MH - Polymerase Chain Reaction/*methods MH - Receptors, Aryl Hydrocarbon/*genetics MH - Soil Pollutants/*analysis EDAT- 2008/03/29 09:00 MHDA- 2008/08/30 09:00 CRDT- 2008/03/29 09:00 PHST- 2007/04/27 00:00 [received] PHST- 2007/08/07 00:00 [revised] PHST- 2007/08/08 00:00 [accepted] PHST- 2008/03/29 09:00 [pubmed] PHST- 2008/08/30 09:00 [medline] PHST- 2008/03/29 09:00 [entrez] AID - S0039-9140(07)00560-7 [pii] AID - 10.1016/j.talanta.2007.08.005 [doi] PST - ppublish SO - Talanta. 2008 Jan 15;74(4):992-7. doi: 10.1016/j.talanta.2007.08.005. Epub 2007 Aug 14.