PMID- 18371866
OWN - NLM
STAT- MEDLINE
DCOM- 20080716
LR  - 20131121
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 75
IP  - 1
DP  - 2008 Mar 15
TI  - Preparation of PVA membrane for immobilization of GOD for glucose biosensor.
PG  - 183-8
LID - 10.1016/j.talanta.2007.10.048 [doi]
AB  - A membrane was prepared using polyvinyl alcohol (PVA) with low and high degree of 
      polymerization (DOP), acetone, benzoic acid (BA) and was cross-linked by UV 
      treatment. Membrane composition was optimized on the basis of swelling index. 
      Membrane prepared with 12% low DOP and 8% high DOP of PVA, 2% BA, dissolved in 
      buffer containing 20% acetone and cross-linked with UV treatment exhibited lower 
      swelling index. Fourier transform infrared (FTIR) study of the membranes showed 
      appearance of a strong band at approximately 2337 cm(-1) when UV was used for 
      cross-linking in the presence of benzoic acid. Scanning electron microscope (SEM) 
      study revealed that membrane cross-linked with UV treatment was smoother. Glucose 
      oxidase (GOD)-PVA membrane was associated with the dissolved oxygen (DO) probe 
      for biosensor reading. Glucose was detected on the basis of depletion of oxygen, 
      when immobilized GOD oxidizes glucose to gluconolactone. A wide detection range, 
      0.9-225 mg/dl was estimated from the linear range of calibration plot of 
      biosensor reading. Membranes were reused for 32 reactions without significant 
      loss of activity and stored for 30 days (approximately 90% activity) at 4 degrees 
      C. Membranes were also used with real blood samples.
FAU - Kumar, Jitendra
AU  - Kumar J
AD  - Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 
      Mumbai 400085, India.
FAU - D'Souza, S F
AU  - D'Souza SF
LA  - eng
PT  - Journal Article
DEP - 20071109
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Blood Glucose)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Membranes, Artificial)
RN  - 9002-89-5 (Polyvinyl Alcohol)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Biosensing Techniques/*instrumentation
MH  - Blood Glucose/analysis
MH  - Enzymes, Immobilized/*chemistry
MH  - Glucose/*chemistry/metabolism
MH  - Glucose Oxidase/*metabolism
MH  - Humans
MH  - Membranes, Artificial
MH  - Polyvinyl Alcohol/*chemistry
MH  - Reproducibility of Results
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Time Factors
EDAT- 2008/03/29 09:00
MHDA- 2008/07/17 09:00
CRDT- 2008/03/29 09:00
PHST- 2007/08/14 00:00 [received]
PHST- 2007/10/29 00:00 [revised]
PHST- 2007/10/29 00:00 [accepted]
PHST- 2008/03/29 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2008/03/29 09:00 [entrez]
AID - S0039-9140(07)00745-X [pii]
AID - 10.1016/j.talanta.2007.10.048 [doi]
PST - ppublish
SO  - Talanta. 2008 Mar 15;75(1):183-8. doi: 10.1016/j.talanta.2007.10.048. Epub 2007 
      Nov 9.