PMID- 18374131
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20211203
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 40
IP  - 2
DP  - 2008 Mar
TI  - Long-term survival of HLA-DR15+ pig skin in SCID mice after reconstitution with 
      human peripheral blood mononuclear cells and under short-term immunosuppression.
PG  - 570-3
LID - 10.1016/j.transproceed.2008.02.006 [doi]
AB  - In allotransplantation, donor-recipient human leukocyte antigen (HLA) matches 
      improve graft survival. For studies of the role of donor-recipient HLA II 
      matching on xenotransplantation, we successfully generated HLA-DR15+ transgenic 
      pigs the the skins of which were transplanted to SCID mice, which were thereafter 
      reconstituted with HLA-DR15+ or -DR15(-) hPBMC. Cyclosporine was given 
      intraperitoneally to SCID mice for 12 days. Human T cells were observed in SCID 
      mice after reconstitution. Mixed lymphocytes responses showed greater responses 
      by HLA-DR15(-) human peripheral blood mononuclear cells (hPBMC) against HLA-DR15+ 
      porcine PBMC. HLA-DR15+ porcine skins survived more than 100 days in all SCID 
      mice. HLA-DR15+ porcine skins were rejected in all non-SCID (Balb/c) mice. The 
      histologic pictures of transplanted HLA-DR15+ porcine skins showed surviving 
      porcine epithelium in remodeling murine dermis and little lymphocyte infiltration 
      into the murine dermis. The long-term survival of HLA-DR15+ pig skin in all 
      hPBMC-SCID mice might be due to poor engraftment or function of reconstituted T 
      cells. Further studies are needed to clarify the role of donor-recipient matching 
      of HLA-DR15.
FAU - Tai, H-C
AU  - Tai HC
AD  - Department of Surgery, National Taiwan University Hospital and College of 
      Medicine, Taipei, Taiwan.
FAU - Tu, C-F
AU  - Tu CF
FAU - Lee, J-M
AU  - Lee JM
FAU - Ho, L-L
AU  - Ho LL
FAU - Tseng, Y-L
AU  - Tseng YL
FAU - Chou, N-K
AU  - Chou NK
FAU - Yang, T-S
AU  - Yang TS
FAU - Weng, C-N
AU  - Weng CN
FAU - Lee, P-H
AU  - Lee PH
FAU - Chang, K-J
AU  - Chang KJ
FAU - Tang, Y-B
AU  - Tang YB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DR Serological Subtypes)
RN  - 0 (HLA-DR15 antigen)
SB  - IM
MH  - Animals
MH  - Graft Survival/*immunology
MH  - HLA-DR Antigens/*immunology
MH  - HLA-DR Serological Subtypes
MH  - Humans
MH  - Immunosuppression Therapy
MH  - Leukocytes, Mononuclear/*immunology
MH  - Mice
MH  - Mice, SCID
MH  - Skin/*immunology
MH  - Skin Transplantation/*immunology
MH  - Swine
EDAT- 2008/04/01 09:00
MHDA- 2008/06/25 09:00
CRDT- 2008/04/01 09:00
PHST- 2008/04/01 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2008/04/01 09:00 [entrez]
AID - S0041-1345(08)00115-2 [pii]
AID - 10.1016/j.transproceed.2008.02.006 [doi]
PST - ppublish
SO  - Transplant Proc. 2008 Mar;40(2):570-3. doi: 10.1016/j.transproceed.2008.02.006.