PMID- 18374609 OWN - NLM STAT- MEDLINE DCOM- 20080903 LR - 20220321 IS - 1095-9564 (Electronic) IS - 1074-7427 (Linking) VI - 90 IP - 1 DP - 2008 Jul TI - Upregulation of hippocampal TrkB and synaptotagmin is involved in treadmill exercise-enhanced aversive memory in mice. PG - 81-9 LID - 10.1016/j.nlm.2008.02.005 [doi] AB - Cognitive functions usually involve various synaptic proteins and neurotrophic factors in the hippocampus. However, whether treadmill exercise can improve learning and memory by upregulating some of these molecules remain unraveled. To address this question, male BALB/c mice were divided into control and exercise groups, the latter group went through 4 weeks of treadmill exercise training. At the end of exercise training period, they were either tested for passive avoidance (PA) performance or sacrificed for quantifying the hippocampal levels of brain-derived neurotrophic factor (BDNF), tropomyosin-related kinase B (TrkB, the BDNF receptor), synaptotagmin (a Ca(2+)-dependent synaptic vesicle protein), and SNAP-25 (a presynaptic vesicular fusion protein). Our results showed that treadmill exercise training (1) increased the retention latency without affecting the fear acquisition in the PA test, (2) transiently increased the hippocampal BDNF level at 1, 2, and 4h after the completion of exercise training, and (3) persistently increased the hippocampal protein levels of full-length TrkB, phosphorylated TrkB and synaptotagmin, but not truncated TrkB or SNAP-25. Moreover, the protein expression level of full-length TrkB or synaptotagmin was positively correlated with PA performance in mice. Finally, inhibition of TrkB signaling by K252a abolished the exercise-facilitated PA performance and upregulation of TrkB and synaptotagmin. Taken together, these data suggest that the upregulation of TrkB and synaptotagmin in the hippocampus contributes to the exercise-facilitated aversive memory. FAU - Liu, Yu-Fan AU - Liu YF AD - Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan. FAU - Chen, Hsiun-ing AU - Chen HI FAU - Yu, Lung AU - Yu L FAU - Kuo, Yu-Min AU - Kuo YM FAU - Wu, Fong-Sen AU - Wu FS FAU - Chuang, Jih-Ing AU - Chuang JI FAU - Liao, Pao-Chi AU - Liao PC FAU - Jen, Chauying J AU - Jen CJ LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20080418 PL - United States TA - Neurobiol Learn Mem JT - Neurobiology of learning and memory JID - 9508166 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Carbazoles) RN - 0 (Enzyme Inhibitors) RN - 0 (Indole Alkaloids) RN - 0 (Membrane Glycoproteins) RN - 0 (Synaptosomal-Associated Protein 25) RN - 0 (Synaptotagmin I) RN - 0 (Syt1 protein, mouse) RN - 97161-97-2 (staurosporine aglycone) RN - EC 2.7.10.1 (Ntrk2 protein, mouse) RN - EC 2.7.10.1 (Protein-Tyrosine Kinases) RN - W980KJ009P (Corticosterone) SB - IM MH - Animals MH - Avoidance Learning/*physiology MH - Brain-Derived Neurotrophic Factor/metabolism MH - Carbazoles/pharmacology MH - Corticosterone/blood MH - Enzyme Inhibitors/pharmacology MH - Hippocampus/*physiology MH - Indole Alkaloids/pharmacology MH - Male MH - Membrane Glycoproteins/antagonists & inhibitors/*metabolism MH - Memory/*physiology MH - Mice MH - Mice, Inbred BALB C MH - Physical Conditioning, Animal/*physiology MH - Protein-Tyrosine Kinases/antagonists & inhibitors/*metabolism MH - Running/physiology MH - Synaptosomal-Associated Protein 25/metabolism MH - Synaptotagmin I/*metabolism MH - Up-Regulation/physiology EDAT- 2008/04/01 09:00 MHDA- 2008/09/04 09:00 CRDT- 2008/04/01 09:00 PHST- 2007/10/17 00:00 [received] PHST- 2008/02/11 00:00 [revised] PHST- 2008/02/15 00:00 [accepted] PHST- 2008/04/01 09:00 [pubmed] PHST- 2008/09/04 09:00 [medline] PHST- 2008/04/01 09:00 [entrez] AID - S1074-7427(08)00032-4 [pii] AID - 10.1016/j.nlm.2008.02.005 [doi] PST - ppublish SO - Neurobiol Learn Mem. 2008 Jul;90(1):81-9. doi: 10.1016/j.nlm.2008.02.005. Epub 2008 Apr 18.