PMID- 18376283
OWN - NLM
STAT- MEDLINE
DCOM- 20080820
LR  - 20111006
IS  - 1077-4114 (Print)
IS  - 1077-4114 (Linking)
VI  - 30
IP  - 3
DP  - 2008 Mar
TI  - Clot strength: a comparison between cord and adult blood by means of 
      thrombelastometry.
PG  - 210-3
LID - 10.1097/MPH.0b013e318162bd2c [doi]
AB  - OBJECTIVE: To evaluate the clot strength in cord versus adult blood. METHOD: 
      Thrombelastometry (TEM) was the method of choice as it provides information on 
      the clot strength in terms of the maximum clot firmness (MCF) and on the fibrin 
      polymerization process in terms of the clot formation time and the alpha angle. 
      RESULTS: The MCFs were significantly lower in cord versus adult platelet rich 
      plasma (PRP, 63.0+/-3.8 vs. 67.0+/-3.9 mm, P=0.006) and in cord versus adult 
      whole blood (WB, 55.3+/-3.8 vs. 59.3+/-3.6 mm, P=0.001) employing the 
      thrombelastometry with extrinsic activator assay. We suggest that the diminished 
      clot strength in cord versus adult blood and plasma samples is attributable to an 
      impaired polymerization of neonatal fibrin: (i) the thrombelastometry with 
      extrinsic activator and inactivated platelets (FIBTEM) assay revealed 
      significantly lower MCFs in cord versus adult PRP (23.0+/-3.1 mm vs. 27.3+/-3.9 
      mm, P=0.002) and in cord versus adult WB (11.6+/-2.3 mm vs. 15.3+/-3.3 mm, 
      P<0.001); (ii) the alpha angle in the FIBTEM assay was significantly lower in 
      cord versus adult WB (39.0+/-12.8 degrees vs. 55.5+/-12.3 degrees, P=0.02); (iii) 
      the clot formation times in the FIBTEM assay were significantly longer in cord 
      versus adult PRP (248.0+/-143.5 s vs. 81.5+/-39.8 s, P=0.001). CONCLUSIONS: 
      Neonatal fibrin shows impaired polymerization properties under our experimental 
      conditions resulting in reduced clot strength compared with fibrin of adult 
      origin.
FAU - Cvirn, Gerhard
AU  - Cvirn G
AD  - Institute of Physiological Chemistry, Department of Pediatrics, Medical 
      University of Graz, Graz, Austria. gerhard.cvirn@meduni-graz.at
FAU - Gallistl, Siegfried
AU  - Gallistl S
FAU - Kutschera, Joerg
AU  - Kutschera J
FAU - Wagner, Thomas
AU  - Wagner T
FAU - Ferstl, Ulrika
AU  - Ferstl U
FAU - Jurgens, Gunther
AU  - Jurgens G
FAU - Koestenberger, Martin
AU  - Koestenberger M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Blood Coagulation/*physiology
MH  - Blood Platelets/physiology
MH  - Elasticity
MH  - Fetal Blood/*physiology
MH  - Humans
MH  - Infant, Newborn
MH  - Reference Values
MH  - Thrombelastography/*methods/*standards
EDAT- 2008/04/01 09:00
MHDA- 2008/08/21 09:00
CRDT- 2008/04/01 09:00
PHST- 2008/04/01 09:00 [pubmed]
PHST- 2008/08/21 09:00 [medline]
PHST- 2008/04/01 09:00 [entrez]
AID - 10.1097/MPH.0b013e318162bd2c [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 2008 Mar;30(3):210-3. doi: 10.1097/MPH.0b013e318162bd2c.