PMID- 18378218
OWN - NLM
STAT- MEDLINE
DCOM- 20081002
LR  - 20210930
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1208
DP  - 2008 May 7
TI  - Transdifferentiation of bone marrow stromal cells into Schwann cell phenotype 
      using progesterone as inducer.
PG  - 17-24
LID - 10.1016/j.brainres.2008.02.071 [doi]
AB  - Bone marrow stromal cells (BMSCs) were reported to transdifferentiate into 
      Schwann cells by a two-stage protocol, using beta-mercaptoethanol and retinoic 
      acid (BME-RA) as preinducers (preinduction stage: PS) and platelet derived growth 
      factor (PDGF), basic fibroblast growth factor (bFGF), forskolin (FSK) and 
      heregulin (HRG) as inducers (induction stage: IS). In this study, six groups were 
      used, group one was used as control (PS: BME-RA; IS: PDGF, bFGF, FSK and HRG). In 
      group 2, the preinducer was similar to group 1, and in the induction stage, 
      progesterone replaced HRG. In groups 3 and 4, the preinducer was progesterone; 
      and at the induction stage, the inducer was similar to groups 1 and 2. 
      Accordingly, in groups 5 and 6, the preinducer was FSK. The immunohistochemical 
      differentiation markers were S-100 and P0, and RT-PCR markers were OCT-4 and P0 
      at the preinduction stage, while at the induction stage P0 and NeuroD were used. 
      The results of the study showed that S-100 was expressed in the groups after the 
      induction stage, however, P0 was not expressed in any group. There was not any 
      significant difference between the percentage of S100 positive cells in the 1st 
      and 2nd groups. P0 was expressed at the mRNA level in the undifferentiated BMSCs 
      and in the 3rd and 4th groups after the preinduction and the induction stages. 
      The conclusion of this study is that progesterone can induce BMSCs into Schwann 
      cell phenotype.
FAU - Movaghar, Bahar
AU  - Movaghar B
AD  - Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Tiraihi, Taki
AU  - Tiraihi T
FAU - Mesbah-Namin, Seyed A
AU  - Mesbah-Namin SA
LA  - eng
PT  - Journal Article
DEP - 20080306
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Fibronectins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Myelin P0 Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuregulin-1)
RN  - 0 (Progestins)
RN  - 0 (S100 Proteins)
RN  - 169238-82-8 (Neurogenic differentiation factor 1)
RN  - 1F7A44V6OU (Colforsin)
RN  - 4G7DS2Q64Y (Progesterone)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cell Transdifferentiation
MH  - Cells, Cultured
MH  - Colforsin/pharmacology
MH  - Fibronectins/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Intercellular Signaling Peptides and Proteins/pharmacology
MH  - Myelin P0 Protein/genetics/metabolism
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Neuregulin-1/pharmacology
MH  - Progesterone/*pharmacology
MH  - Progestins/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - S100 Proteins/metabolism
MH  - Schwann Cells/*cytology/metabolism
MH  - Stromal Cells/cytology/metabolism
EDAT- 2008/04/02 09:00
MHDA- 2008/10/03 09:00
CRDT- 2008/04/02 09:00
PHST- 2007/09/12 00:00 [received]
PHST- 2008/02/07 00:00 [revised]
PHST- 2008/02/18 00:00 [accepted]
PHST- 2008/04/02 09:00 [pubmed]
PHST- 2008/10/03 09:00 [medline]
PHST- 2008/04/02 09:00 [entrez]
AID - S0006-8993(08)00461-7 [pii]
AID - 10.1016/j.brainres.2008.02.071 [doi]
PST - ppublish
SO  - Brain Res. 2008 May 7;1208:17-24. doi: 10.1016/j.brainres.2008.02.071. Epub 2008 
      Mar 6.