PMID- 18378582
OWN - NLM
STAT- MEDLINE
DCOM- 20080731
LR  - 20220408
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 49
IP  - 7
DP  - 2008 Jul
TI  - Interleukin-6 as a photoreceptor neuroprotectant in an experimental model of 
      retinal detachment.
PG  - 3193-200
LID - 10.1167/iovs.07-1641 [doi]
AB  - PURPOSE: To test the hypothesis that interleukin (IL)-6 prevents photoreceptor 
      cell death during periods of retinal separation from the retinal pigment 
      epithelium (RPE). METHODS: Retinal-RPE separation was created in wild-type C57BL 
      mice, IL-6(-/-) mice, and Brown Norway rats by subretinal injection of 1% 
      hyaluronic acid. In some animals, anti-IL-6 neutralizing antibody (NAB) or 
      exogenous IL-6 was administered into the subretinal space at the time of 
      separation or at specified times afterward. Terminal deoxynucleotidyl transferase 
      dUTP nick-end labeling (TUNEL) was performed 3 days after separation to detect 
      apoptotic photoreceptors. Photoreceptor cell counts were performed after 1 and 2 
      months. RESULTS: Loss of IL-6 function through genetic ablation (IL-6(-/-) mice) 
      or injection of anti-IL-6 NAB resulted in significantly increased levels of 
      TUNEL-positive staining 3 days after retinal-RPE separation. One month after 
      separation, outer nuclear layer (ONL) cell counts were significantly lower in 
      IL-6(-/-) mice or in animals injected with anti-IL-6 NAB than in controls. Gain 
      of IL-6 function through the addition of exogenous IL-6 resulted in significantly 
      increased ONL counts at 1 month but not at 2 months. Reinjection of IL-6 at 1 
      month led to continued preservation of ONL counts compared with controls. A 
      window of opportunity for treatment was detected because delaying injection of 
      exogenous IL-6 to 2 weeks after retinal-RPE separation still resulted in 
      significantly greater ONL cell counts compared with controls. CONCLUSIONS: IL-6 
      may serve as a photoreceptor neuroprotectant in the setting of retinal-RPE 
      separation.
FAU - Chong, Deborah Y
AU  - Chong DY
AD  - Department of Ophthalmology and Visual Sciences, W. K. Kellogg Eye Center, 
      University of Michigan, Ann Arbor, Michigan 48105-0714, USA.
FAU - Boehlke, Christopher S
AU  - Boehlke CS
FAU - Zheng, Qiong-Duan
AU  - Zheng QD
FAU - Zhang, Linda
AU  - Zhang L
FAU - Han, Ying
AU  - Han Y
FAU - Zacks, David N
AU  - Zacks DN
LA  - eng
GR  - EY-07003/EY/NEI NIH HHS/United States
GR  - K08-124-14705/PHS HHS/United States
GR  - F31 EY007003/EY/NEI NIH HHS/United States
GR  - K08 EY014705/EY/NEI NIH HHS/United States
GR  - P30 EY007003/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080331
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Antibodies)
RN  - 0 (Interleukin-6)
RN  - 0 (Neuroprotective Agents)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Count
MH  - Drug Administration Schedule
MH  - Humans
MH  - Interleukin-6/administration & dosage/deficiency/immunology/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Neuroprotective Agents/administration & dosage/antagonists & 
      inhibitors/metabolism/*pharmacology
MH  - Photoreceptor Cells, Vertebrate/*drug effects/metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - Retina/drug effects/pathology
MH  - Retinal Detachment/pathology/*physiopathology
MH  - Time Factors
PMC - PMC3704181
MID - NIHMS488080
EDAT- 2008/04/02 09:00
MHDA- 2008/08/01 09:00
PMCR- 2013/07/08
CRDT- 2008/04/02 09:00
PHST- 2008/04/02 09:00 [pubmed]
PHST- 2008/08/01 09:00 [medline]
PHST- 2008/04/02 09:00 [entrez]
PHST- 2013/07/08 00:00 [pmc-release]
AID - iovs.07-1641 [pii]
AID - 10.1167/iovs.07-1641 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2008 Jul;49(7):3193-200. doi: 10.1167/iovs.07-1641. 
      Epub 2008 Mar 31.