PMID- 18381233
OWN - NLM
STAT- MEDLINE
DCOM- 20090421
LR  - 20220318
IS  - 1872-6623 (Electronic)
IS  - 0304-3959 (Print)
IS  - 0304-3959 (Linking)
VI  - 138
IP  - 3
DP  - 2008 Sep 15
TI  - A-kinase anchoring protein mediates TRPV1 thermal hyperalgesia through PKA 
      phosphorylation of TRPV1.
PG  - 604-616
LID - 10.1016/j.pain.2008.02.022 [doi]
AB  - Certain phosphorylation events are tightly controlled by scaffolding proteins 
      such as A-kinase anchoring protein (AKAP). On nociceptive terminals, 
      phosphorylation of transient receptor potential channel type 1 (TRPV1) results in 
      the sensitization to many different stimuli, contributing to the development of 
      hyperalgesia. In this study, we investigated the functional involvement of 
      AKAP150 in mediating sensitization of TRPV1, and found that AKAP150 is 
      co-expressed in trigeminal ganglia (TG) neurons from rat and associates with 
      TRPV1. Furthermore, siRNA-mediated knock-down of AKAP150 expression led to a 
      significant reduction in PKA phosphorylation of TRPV1 in cultured TG neurons. In 
      CHO cells, the PKA RII binding site on AKAP was necessary for PKA enhancement of 
      TRPV1-mediated Ca2+-accumulation. In addition, AKAP150 knock-down in cultured TG 
      neurons attenuated PKA sensitization of TRPV1 activity and in vivo administration 
      of an AKAP antagonist significantly reduced prostaglandin E2 sensitization to 
      thermal stimuli. These data suggest that AKAP150 functionally regulates 
      PKA-mediated phosphorylation/sensitization of the TRPV1 receptor.
FAU - Jeske, Nathaniel A
AU  - Jeske NA
AD  - Department of Oral and Maxillofacial Surgery, University of Texas Health Science 
      Center of San Antonio, MC 7908, 7703 Floyd Curl Dr., San Antonio, TX 78229-3900, 
      USA Department of Endodontics, University of Texas Health Science Center of San 
      Antonio, San Antonio, TX, USA Department of Cellular and Structural Biology, 
      University of Texas Health Science Center of San Antonio, San Antonio, TX, USA 
      Department of Pharmacology, University of Texas Health Science Center of San 
      Antonio, San Antonio, TX, USA.
FAU - Diogenes, Anibal
AU  - Diogenes A
FAU - Ruparel, Nikita B
AU  - Ruparel NB
FAU - Fehrenbacher, Jill C
AU  - Fehrenbacher JC
FAU - Henry, Michael
AU  - Henry M
FAU - Akopian, Armen N
AU  - Akopian AN
FAU - Hargreaves, Kenneth M
AU  - Hargreaves KM
LA  - eng
GR  - DE13942/DE/NIDCR NIH HHS/United States
GR  - DE016500/DE/NIDCR NIH HHS/United States
GR  - F32 DE016500-02/DE/NIDCR NIH HHS/United States
GR  - F32 DE016500/DE/NIDCR NIH HHS/United States
GR  - DA19585/DA/NIDA NIH HHS/United States
GR  - R01 DA019585/DA/NIDA NIH HHS/United States
GR  - R01 DE013942/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080401
PL  - United States
TA  - Pain
JT  - Pain
JID - 7508686
RN  - 0 (A Kinase Anchor Proteins)
RN  - 0 (Akap5 protein, rat)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV1 receptor)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
SB  - IM
MH  - A Kinase Anchor Proteins/deficiency/genetics/*metabolism/physiology
MH  - Animals
MH  - CHO Cells
MH  - Cells, Cultured
MH  - Cricetinae
MH  - Cricetulus
MH  - Cyclic AMP-Dependent Protein Kinases/genetics/*metabolism
MH  - Guinea Pigs
MH  - Hyperalgesia/enzymology/*metabolism
MH  - Neurons/enzymology/metabolism
MH  - Phosphorylation
MH  - RNA, Small Interfering/physiology
MH  - Rats
MH  - TRPV Cation Channels/antagonists & inhibitors/genetics/*metabolism
MH  - Trigeminal Ganglion/cytology/enzymology/metabolism
PMC - PMC2593399
MID - NIHMS71597
EDAT- 2008/04/03 09:00
MHDA- 2009/04/22 09:00
PMCR- 2009/09/15
CRDT- 2008/04/03 09:00
PHST- 2007/09/24 00:00 [received]
PHST- 2007/12/17 00:00 [revised]
PHST- 2008/02/20 00:00 [accepted]
PHST- 2008/04/03 09:00 [pubmed]
PHST- 2009/04/22 09:00 [medline]
PHST- 2008/04/03 09:00 [entrez]
PHST- 2009/09/15 00:00 [pmc-release]
AID - 00006396-200809150-00017 [pii]
AID - 10.1016/j.pain.2008.02.022 [doi]
PST - ppublish
SO  - Pain. 2008 Sep 15;138(3):604-616. doi: 10.1016/j.pain.2008.02.022. Epub 2008 Apr 
      1.