PMID- 18384885
OWN - NLM
STAT- MEDLINE
DCOM- 20080725
LR  - 20080408
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 117
IP  - 2
DP  - 2008 May 15
TI  - Human and murine model cell lines for dendritic cell biology evaluated.
PG  - 191-7
LID - 10.1016/j.imlet.2008.02.003 [doi]
AB  - Dendritic cells (DCs) are specialized antigen presenting cells that link innate 
      and adaptive immune responses. As key mediators of T cell dependent immunity, DCs 
      are considered primary targets for initiating immune responses in infectious 
      diseases and cancer. Conversely, DCs can also play an important role in the 
      induction of tolerance in organ transplantation, autoimmune disorders and 
      allergy. While DCs have been used in clinical trials worldwide during the past 
      decade, many of the highly specialized cell biological characteristics of DCs 
      remain poorly understood. Small numbers of DCs can be isolated as terminally 
      differentiated, post-mitotic cells form either blood or spleen. Alternatively, 
      DC-precursors, such as monocytes or bone marrow-derived stem cells, can be 
      isolated and differentiated into DCs in vitro. The relative low numbers of cells 
      that can thus be obtained, combined with difficulties manipulating these 
      terminally differentiated primary cells in vitro and in vivo, have seriously 
      hampered studies aimed at exploring the cell biology of DCs. Good model cell 
      lines therefore provide invaluable tools to study DC biology. So far most DC 
      models are myeloid leukemia-derived cell lines that can be differentiated in 
      vitro towards a DC phenotype. Here, we compared the phenotypical and functional 
      characteristics of frequently used mouse and human DC-model cell lines. We 
      conclude that, although none of these cell lines fully recapitulates all cell 
      biological or immunological features of primary DCs, some of these cell lines 
      provide valuable tools to study specific aspects of DC biology.
FAU - van Helden, Suzanne F G
AU  - van Helden SF
AD  - Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University Nijmegen Medical Centre, Geert Grooteplein Zuid 28, PO Box 
      9101, 6500 HB Nijmegen, The Netherlands.
FAU - van Leeuwen, Frank N
AU  - van Leeuwen FN
FAU - Figdor, Carl G
AU  - Figdor CG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20080311
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Differentiation
MH  - Cell Adhesion
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - *Cell Line
MH  - Cell Movement
MH  - *Dendritic Cells/immunology/metabolism/pathology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Mice
MH  - Species Specificity
RF  - 79
EDAT- 2008/04/04 09:00
MHDA- 2008/07/26 09:00
CRDT- 2008/04/04 09:00
PHST- 2008/01/24 00:00 [received]
PHST- 2008/02/05 00:00 [revised]
PHST- 2008/02/08 00:00 [accepted]
PHST- 2008/04/04 09:00 [pubmed]
PHST- 2008/07/26 09:00 [medline]
PHST- 2008/04/04 09:00 [entrez]
AID - S0165-2478(08)00044-8 [pii]
AID - 10.1016/j.imlet.2008.02.003 [doi]
PST - ppublish
SO  - Immunol Lett. 2008 May 15;117(2):191-7. doi: 10.1016/j.imlet.2008.02.003. Epub 
      2008 Mar 11.