PMID- 18385439
OWN - NLM
STAT- MEDLINE
DCOM- 20080723
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 46
IP  - 6
DP  - 2008 Jun
TI  - Identification of thermotolerant campylobacter species by fluorescence in situ 
      hybridization.
PG  - 2133-6
LID - 10.1128/JCM.01512-07 [doi]
AB  - Thermotolerant Campylobacter spp. (Campylobacter jejuni, C. coli, C. lari, and C. 
      upsaliensis) are leading causes of food-borne diarrhea in humans. In this study, 
      the usefulness of fluorescence in situ hybridization (FISH) for the 
      identification of Campylobacter isolates was investigated. A hierarchical FISH 
      probe set that included six group-, genus-, and species-specific probes was 
      developed and evaluated with 12 reference strains and 94 clinical isolates of 
      Campylobacter, Arcobacter, and Helicobacter. FISH correctly identified all 
      isolates to the genus level and detected all thermotolerant Campylobacter 
      isolates. The assay showed high degrees of sensitivity for the identification of 
      C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the 
      species level.
FAU - Poppert, Sven
AU  - Poppert S
AD  - Institute of Medical Microbiology and Hygiene, University of Ulm, Ulm, Germany. 
      poppert@bni-hamburg.de
FAU - Haas, Michaela
AU  - Haas M
FAU - Yildiz, Tatjana
AU  - Yildiz T
FAU - Alter, Thomas
AU  - Alter T
FAU - Bartel, Edda
AU  - Bartel E
FAU - Fricke, Ursula
AU  - Fricke U
FAU - Essig, Andreas
AU  - Essig A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080402
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Culture Media)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Bacterial Typing Techniques
MH  - Campylobacter/*classification/genetics/isolation & purification
MH  - Culture Media
MH  - DNA Probes
MH  - *Hot Temperature
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Species Specificity
PMC - PMC2446828
EDAT- 2008/04/04 09:00
MHDA- 2008/07/24 09:00
PMCR- 2008/10/01
CRDT- 2008/04/04 09:00
PHST- 2008/04/04 09:00 [pubmed]
PHST- 2008/07/24 09:00 [medline]
PHST- 2008/04/04 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - JCM.01512-07 [pii]
AID - 1512-07 [pii]
AID - 10.1128/JCM.01512-07 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2008 Jun;46(6):2133-6. doi: 10.1128/JCM.01512-07. Epub 2008 Apr 
      2.