PMID- 18385909
OWN - NLM
STAT- MEDLINE
DCOM- 20080818
LR  - 20211020
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 10
IP  - 3
DP  - 2008 May
TI  - Detection of TMPRSS2:ERG fusion gene in circulating prostate cancer cells.
PG  - 467-73
LID - 10.1111/j.1745-7262.2008.00401.x [doi]
AB  - AIM: To investigate the existence of TMPRSS2:ERG fusion gene in circulating tumor 
      cells (CTC) from prostate cancer patients and its potential in monitoring tumor 
      metastasis. METHODS: We analyzed the frequency of TMPRSS2:ERG and TMPRSS2:ETV1 
      transcripts in 27 prostate cancer biopsies from prostatectomies, and TMPRSS2:ERG 
      transcripts in CTC isolated from 15 patients with advanced androgen independent 
      disease using reverse transcription polymerase chain reaction (RT-PCR). 
      Fluorescence in situ hybridization (FISH) was applied to analyze the genomic 
      truncation of ERG, which is the result of TMPRSS2:ERG fusion in 10 of the 15 CTC 
      samples. RESULTS: TMPRSS2:ERG transcripts were found in 44% of our samples, but 
      we did not detect expression of TMPRSS2:ETV1. Using FISH analysis we detected 
      chromosomal rearrangements affecting the ERG gene in 6 of 10 CTC samples, 
      including 1 case with associated TMPRSS2:ERG fusion at the primary site. However, 
      TMPRSS2:ERG transcripts were not detected in any of the 15 CTC samples, including 
      the 10 cases analyzed by FISH. CONCLUSION: Although further study is required to 
      address the association between TMPRSS2:ERG fusion and prostate cancer 
      metastasis, detection of genomic truncation of the ERG gene by FISH analysis 
      could be useful for monitoring the appearance of CTC and the potential for 
      prostate cancer metastasis.
CI  - 2008, Asian Journal of Andrology, SIMM and SJTU. All rights reserved.
FAU - Mao, Xueying
AU  - Mao X
AD  - Medical Oncology Centre, Cancer Institute, Department of Histopathology and 
      Morbid Anatomy, Barts and London School of Medicine and Dentistry, Queen Mary, 
      University of London, Charterhouse Square, London EC1M 6BQ, UK.
FAU - Shaw, Greg
AU  - Shaw G
FAU - James, Sharon Y
AU  - James SY
FAU - Purkis, Patricia
AU  - Purkis P
FAU - Kudahetti, Sakunthala C
AU  - Kudahetti SC
FAU - Tsigani, Theodora
AU  - Tsigani T
FAU - Kia, Saname
AU  - Kia S
FAU - Young, Bryan D
AU  - Young BD
FAU - Oliver, R Tim D
AU  - Oliver RT
FAU - Berney, Dan
AU  - Berney D
FAU - Prowse, David M
AU  - Prowse DM
FAU - Lu, Yong-Jie
AU  - Lu YJ
LA  - eng
GR  - A6438/CRUK_/Cancer Research UK/United Kingdom
GR  - A6789/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (DNA Primers)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TMPRSS2-ERG fusion protein, human)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Neoplastic Cells, Circulating
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Prostatic Neoplasms/blood/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/04/04 09:00
MHDA- 2008/08/19 09:00
CRDT- 2008/04/04 09:00
PHST- 2008/04/04 09:00 [pubmed]
PHST- 2008/08/19 09:00 [medline]
PHST- 2008/04/04 09:00 [entrez]
AID - 10.1111/j.1745-7262.2008.00401.x [doi]
PST - ppublish
SO  - Asian J Androl. 2008 May;10(3):467-73. doi: 10.1111/j.1745-7262.2008.00401.x.