PMID- 1839143
OWN - NLM
STAT- MEDLINE
DCOM- 19920415
LR  - 20190705
IS  - 0009-2363 (Print)
IS  - 0009-2363 (Linking)
VI  - 39
IP  - 8
DP  - 1991 Aug
TI  - Effects of metal elements on beta-hexosaminidase release from rat basophilic 
      leukemia cells (RBL-2H3).
PG  - 2072-6
AB  - Immediate allergy is caused by a chemical mediator released from basophile and 
      mast cells via cell degranulation due to reaction between an immunoglobulin E 
      (IgE) antibody, bound with the IgE receptor on the cell membrane, and an antigen. 
      The present authors have established a new method for assaying the enzyme 
      activity of beta-hexosaminidase as an index of chemical mediator release. Using 
      cultured cells instead of conventional methods based on histamine release from 
      mast cells, the present method permits highly accurate mass screening since it 
      uses a well-established cell line of rat basophilic leukemia cells (RBL-2H3). The 
      effects of metal elements on immediate allergic reaction were evaluated using a 
      newly developed assay system. A total of 38 metal elements were investigated for 
      effects on immediate allergic reactions in vitro. These elements were classified 
      by five types on the basis of action on beta-hexosanimidase release: 1) those 
      which showed very strong inhibitory action, such as ZnCl2 and ZrCl4, 2) those 
      which showed relatively strong inhibitory action, such as CdCl2 and CuCl2, 3) 
      those which showed relatively weak inhibitory action, such as CoCl2 and Pb(NO3)2, 
      4) those which showed neither inhibitory nor promoting action, such as MnCl2 and 
      SrCl2, and 5) AgNO3, which alone showed promoting action.
FAU - Tanaka, Y
AU  - Tanaka Y
AD  - Osaka Prefectural Institute of Public Health, Japan.
FAU - Takagaki, Y
AU  - Takagaki Y
FAU - Nishimune, T
AU  - Nishimune T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Metals)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
SB  - IM
MH  - Animals
MH  - Basophils/drug effects/*enzymology
MH  - Leukemia, Experimental/*enzymology
MH  - Metals/*pharmacology
MH  - Rats
MH  - Tumor Cells, Cultured/drug effects/enzymology
MH  - beta-N-Acetylhexosaminidases/*metabolism
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - 10.1248/cpb.39.2072 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 1991 Aug;39(8):2072-6. doi: 10.1248/cpb.39.2072.