PMID- 18391576
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20181201
IS  - 1423-0275 (Electronic)
IS  - 0301-1569 (Linking)
VI  - 70
IP  - 3
DP  - 2008
TI  - Suppression of survivin expression by short hairpin RNA induces apoptosis in 
      human laryngeal carcinoma cells.
PG  - 168-75
LID - 10.1159/000124290 [doi]
AB  - PURPOSE: The aim of this study was to evaluate the suppression effect of survivin 
      shRNA on the expression of the survivin gene in the human laryngeal cancer cell 
      line Hep-2. PROCEDURES: 60 cases of laryngeal squamous-cell carcinoma (LSCC) and 
      10 cases of normal laryngeal mucosa were examined using immunohistochemistry to 
      determine whether the expression of survivin correlated with tumorigenesis. Three 
      plasmid vectors of short hairpin RNA (shRNA) specific for survivin were designed 
      and generated. Western blot and real-time PCR analysis of survivin expression in 
      Hep-2 cells was performed 48 h after transfection. The growth curve was used to 
      determine the cell proliferation. Propidium iodide (PI) single staining was 
      applied to detect the cell cycle. The apoptosis of the cells was analyzed by flow 
      cytometry with the FITC-annexin-V/PI double staining and PI single staining. 
      RESULTS: 68.33% (41 out of 60) of tumors were positive for survivin expression 
      and significantly associated with lymph node metastasis and advanced stage. In 
      contrast, no expression of survivin in normal mucosa was detected. Transfection 
      of Hep-2 cells with survivin shRNA significantly inhibited survivin expression at 
      both the mRNA and the protein level in Hep-2 cells. Downregulation of survivin 
      resulted in increasing the apoptosis index, but the results showed no obvious 
      influence on cell cycle. CONCLUSIONS: This study demonstrates that survivin shRNA 
      effectively inhibits survivin gene expression in Hep-2 cells leading to growth 
      suppression and apoptotic induction in Hep-2 cells.
CI  - 2008 S. Karger AG, Basel
FAU - Chen, Xiu-mei
AU  - Chen XM
AD  - Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Jinan, 
      China.
FAU - Luan, Xin-yong
AU  - Luan XY
FAU - Lei, Da-peng
AU  - Lei DP
FAU - Ma, Xiao-jie
AU  - Ma XJ
FAU - Liu, Xue-xia
AU  - Liu XX
FAU - Liu, Jie
AU  - Liu J
FAU - Pan, Xin-liang
AU  - Pan XL
LA  - eng
PT  - Journal Article
DEP - 20080408
PL  - Switzerland
TA  - ORL J Otorhinolaryngol Relat Spec
JT  - ORL; journal for oto-rhino-laryngology and its related specialties
JID - 0334721
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Survivin)
SB  - IM
MH  - *Apoptosis
MH  - Carcinoma, Squamous Cell/etiology/metabolism/pathology/*physiopathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Immunohistochemistry
MH  - Inhibitor of Apoptosis Proteins
MH  - Laryngeal Neoplasms/etiology/metabolism/pathology/*physiopathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Microtubule-Associated Proteins/*antagonists & inhibitors/*genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Proteins/*antagonists & inhibitors/*genetics/metabolism
MH  - *RNA Interference
MH  - RNA, Messenger/antagonists & inhibitors
MH  - Survivin
EDAT- 2008/04/09 09:00
MHDA- 2008/06/25 09:00
CRDT- 2008/04/09 09:00
PHST- 2006/12/13 00:00 [received]
PHST- 2007/06/11 00:00 [accepted]
PHST- 2008/04/09 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2008/04/09 09:00 [entrez]
AID - 000124290 [pii]
AID - 10.1159/000124290 [doi]
PST - ppublish
SO  - ORL J Otorhinolaryngol Relat Spec. 2008;70(3):168-75. doi: 10.1159/000124290. 
      Epub 2008 Apr 8.