PMID- 18392937
OWN - NLM
STAT- MEDLINE
DCOM- 20080716
LR  - 20131121
IS  - 1573-675X (Electronic)
IS  - 1360-8185 (Linking)
VI  - 13
IP  - 5
DP  - 2008 May
TI  - Fetal bovine serum simultaneously stimulates apoptosis and DNA synthesis in 
      premeiotic stages of spermatogenesis in spiny dogfish (Squalus acanthias) in 
      vitro: modulation by androgen and spermatogenic activity status.
PG  - 649-58
LID - 10.1007/s10495-008-0205-x [doi]
AB  - Using the simple cystic spermatogenesis in the shark testis as a model, we 
      previously reported the relative resistance of immature spermatogonia (stem cell 
      and early-stage spermatogonia) to apoptosis in the normal testis and after 
      spermatoxicant exposure in vivo. Apoptosis was monitored by fluorescence image 
      analysis of living cysts, using the validated acridine orange (AO) vital staining 
      technique. Findings show that FBS simultaneously stimulates both apoptosis and 
      [(3)H]thymidine incorporation in immature spermatogonial clones in a 
      concentration-dependent manner in vitro. Furthermore, androgen inhibits apoptosis 
      and increases cyst viability, more so with 10% FBS than with 1% FBS. All the 
      effects were as a function of spermatogenic activity status but were distinct in 
      early-stage spermatogonial cysts isolated from testes awakening from the previous 
      winter spermatogenic arrest period. Results are discussed in the context of the 
      alternating germ-Sertoli cell population kinetics of early-stage spermatogonial 
      cysts in Squalus acanthias's protracted testicular cycle.
FAU - McClusky, Leon Mendel
AU  - McClusky LM
AD  - Department of Health and Nursing Science, Narvik University College, P.O. Box 
      385, Narvik 8505, Norway. leonmc@hin.no
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Apoptosis
JT  - Apoptosis : an international journal on programmed cell death
JID - 9712129
RN  - 08J2K08A3Y (Dihydrotestosterone)
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9007-49-2 (DNA)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cattle
MH  - DNA/*biosynthesis
MH  - Dihydrotestosterone/pharmacology
MH  - Estradiol/pharmacology
MH  - Fetal Blood/*physiology
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Seasons
MH  - Spermatogenesis/drug effects/*physiology
MH  - Squalus acanthias
MH  - Testosterone/pharmacology
MH  - Thymidine/metabolism
EDAT- 2008/04/09 09:00
MHDA- 2008/07/17 09:00
CRDT- 2008/04/09 09:00
PHST- 2008/04/09 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2008/04/09 09:00 [entrez]
AID - 10.1007/s10495-008-0205-x [doi]
PST - ppublish
SO  - Apoptosis. 2008 May;13(5):649-58. doi: 10.1007/s10495-008-0205-x.