PMID- 18393244
OWN - NLM
STAT- MEDLINE
DCOM- 20090319
LR  - 20080408
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 25
IP  - 2
DP  - 2008 Apr
TI  - [Molecular cytogenetic studies of 25 males with azoospermia].
PG  - 190-4
AB  - OBJECTIVE: To investigate the clinical correlation of chromosome abnormalities 
      and microdeletion in azoospermic factor (AZF) region on Y chromosome in 25 
      patients with azoospermia. METHODS: Chromosome analyses were performed by using 
      chromosome GTG-banding, Q-banding, fluorescence in situ hybridization (FISH) and 
      polymerase chain reaction (PCR) for AZF region on chromosome Yq. RESULTS: Seven 
      cases showed abnormal chromosome karyotype (28%). In 8 azoospermic patients 
      tested, 2 showed microdeletions of AZFb (SY127, SY134)+ AZFc (SY254, SY255) and 
      AZFc(SY243, SY158) on chromosome Yq, respectively. CONCLUSION: Chromosome 
      abnormalities and AZF microdeletion are major cause of azoospermia leading to 
      male infertility; male with azoospermia and infertility should be referred to 
      cytogenetic diagnosis by using chromosome GTG-banding, Q-banding after ruling out 
      clinical factors including testopathy, obstructive azoospermia, and abnormalities 
      in incretion and immune system. FISH or PCR analysis for AZF region on chromosome 
      Yq should be done for the patient with azoospermia if Q-banding indicates the 
      deletion above Yq12 region. It is of essential importance to provide precise 
      diagnosis in genetic counseling for further clinical treatment.
FAU - Ge, Yun-sheng
AU  - Ge YS
AD  - Center for Prenatal Diagnosis, Xiamen Maternity & Child Health Care Hospital, 
      Xiamen, Fujian 361003 P. R. China. ljhdm2000@yahoo.com.cn.
FAU - Zhou, Yu-lin
AU  - Zhou YL
FAU - Wu, Hui-nan
AU  - Wu HN
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Azoospermia/*genetics
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Y/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
EDAT- 2008/04/09 09:00
MHDA- 2009/03/20 09:00
CRDT- 2008/04/09 09:00
PHST- 2008/04/09 09:00 [pubmed]
PHST- 2009/03/20 09:00 [medline]
PHST- 2008/04/09 09:00 [entrez]
AID - 940625041 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Apr;25(2):190-4.