PMID- 18393295
OWN - NLM
STAT- MEDLINE
DCOM- 20080801
LR  - 20211020
IS  - 1096-9861 (Electronic)
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 508
IP  - 5
DP  - 2008 Jun 10
TI  - Nucleus paragigantocellularis afferents in male and female rats: organization, 
      gonadal steroid receptor expression, and activation during sexual behavior.
PG  - 771-94
LID - 10.1002/cne.21704 [doi]
AB  - The supraspinal regulation of genital reflexes is poorly understood. The 
      brainstem nucleus paragigantocellularis (nPGi) of rats is a well-established 
      source of tonic inhibition of genital reflexes. However, the organization, 
      gonadal steroid receptor expression, and activity of nPGi afferents during sex 
      have not been fully characterized in male and female rats. To delineate the 
      anatomical and physiological organization of nPGi afferents, the retrograde 
      tracer Fluoro-Gold (FG) was injected into the nPGi of sexually experienced male 
      and female rats. Animals engaged in sexual behavior 1 hour before sacrifice. 
      Cells containing FG, estrogen receptor-alpha (ER(alpha)), androgen receptor (AR), 
      and the immediate-early gene product Fos were identified immunocytochemically. 
      Retrograde labeling from the nPGi was prominent in the bed nucleus of the stria 
      terminalis, paraventricular nucleus (PVN), posterior hypothalamus, precommissural 
      nucleus, deep mesencephalic nucleus, and periaqueductal gray (PAG) of both sexes. 
      Sex differences were observed in the caudal medial preoptic area (MPO), with 
      significantly more FG+ cells observed in males, and in the PAG and inferior 
      colliculus, where significantly more FG+ cells were observed in females. The 
      majority of regions that contained FG+ cells also contained ER(alpha) or AR, 
      indicating sensitivity to gonadal steroids. The proportions of FG+ cells that 
      co-localized with sex-induced Fos was high in the PVN of both sexes and high in 
      the MPO of males but low in the PAG of both sexes despite the large number of 
      PAG-nPGi output neurons and Fos+ cells in both sexes. The characterization of 
      these afferents will lead to a further understanding of the neural regulation of 
      genital reflexes.
FAU - Normandin, Joseph J
AU  - Normandin JJ
AD  - Center for Behavioral Neuroscience, Department of Biology, Georgia State 
      University, Atlanta, Georgia 30303, USA.
FAU - Murphy, Anne Z
AU  - Murphy AZ
LA  - eng
GR  - R01 MH059187-04/MH/NIMH NIH HHS/United States
GR  - R01 DA016272/DA/NIDA NIH HHS/United States
GR  - R01 MH059187-01/MH/NIMH NIH HHS/United States
GR  - R01 MH059187-02/MH/NIMH NIH HHS/United States
GR  - R01 MH059187-03/MH/NIMH NIH HHS/United States
GR  - R01 DA016272-02/DA/NIDA NIH HHS/United States
GR  - R01 DA016272-03/DA/NIDA NIH HHS/United States
GR  - R01 DA016272-01/DA/NIDA NIH HHS/United States
GR  - DA16272/DA/NIDA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Receptors, Steroid)
SB  - IM
MH  - Afferent Pathways/chemistry/physiology
MH  - Animals
MH  - Brain Stem/chemistry/*physiology
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Gonadal Steroid Hormones/analysis/biosynthesis/genetics
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Steroid/*biosynthesis/genetics/physiology
MH  - *Sex Characteristics
MH  - Sexual Behavior, Animal/*physiology
PMC - PMC2823478
MID - NIHMS175634
EDAT- 2008/04/09 09:00
MHDA- 2008/08/02 09:00
PMCR- 2010/02/17
CRDT- 2008/04/09 09:00
PHST- 2008/04/09 09:00 [pubmed]
PHST- 2008/08/02 09:00 [medline]
PHST- 2008/04/09 09:00 [entrez]
PHST- 2010/02/17 00:00 [pmc-release]
AID - 10.1002/cne.21704 [doi]
PST - ppublish
SO  - J Comp Neurol. 2008 Jun 10;508(5):771-94. doi: 10.1002/cne.21704.