PMID- 18396435
OWN - NLM
STAT- MEDLINE
DCOM- 20080728
LR  - 20211020
IS  - 1286-4579 (Print)
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 10
IP  - 4
DP  - 2008 Apr
TI  - Infection of the tracheal epithelium by infectious bronchitis virus is sialic 
      acid dependent.
PG  - 367-73
LID - 10.1016/j.micinf.2007.12.009 [doi]
AB  - Avian Infectious bronchitis virus (IBV) is a coronavirus that infects chickens 
      via the respiratory epithelium as primary target cells. The binding of 
      coronaviruses to the cell surface is mediated by the viral surface protein S. 
      Recently we demonstrated that alpha2,3-linked sialic acid serves as a receptor 
      determinant for IBV on Vero cells and primary chicken embryo kidney cells. Here 
      we analyze the importance of the sialic acid binding activity for the infection 
      of tracheal organ cultures (TOCs) by different IBV strains. Our results show that 
      alpha2,3-linked sialic acid also serves as a receptor determinant on chicken 
      TOCs. Infection of TOCs by IBV results in ciliostasis. Desialylation induced by 
      neuraminidase treatment of tracheal organ cultures prior to infection by IBV 
      delayed the ciliostatic effect or resulted in partial loss of ciliary activity. 
      This effect was observed with both respiratory and nephropathogenic strains. 
      Inhibition of ciliostasis was also observed when TOCs were pretreated with an 
      alpha2,3-specific neuraminidase. Analysis of the tracheal epithelium for 
      reactivity with lectins revealed that the susceptible cells in the epithelium 
      abundantly express alpha2,3-linked sialic acid. These results indicate that 
      alpha2,3-linked sialic acid plays an important role for infection of the 
      respiratory epithelium by IBV.
FAU - Winter, Christine
AU  - Winter C
AD  - Institute of Virology, University of Veterinary Medicine, Bunteweg 17, 30559 
      Hannover, Germany. christine.winter@tiho-hannover.de
FAU - Herrler, Georg
AU  - Herrler G
FAU - Neumann, Ulrich
AU  - Neumann U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20071228
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Receptors, Virus)
RN  - EC 3.2.1.18 (Neuraminidase)
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Animals
MH  - Chick Embryo
MH  - Cilia/physiology
MH  - Infectious bronchitis virus/*physiology
MH  - N-Acetylneuraminic Acid/*metabolism
MH  - Neuraminidase/metabolism
MH  - Organ Culture Techniques
MH  - Receptors, Virus/*metabolism
MH  - Respiratory Mucosa/*virology
MH  - Trachea/virology
MH  - *Virus Attachment
PMC - PMC7110564
EDAT- 2008/04/09 09:00
MHDA- 2008/07/29 09:00
PMCR- 2007/12/28
CRDT- 2008/04/09 09:00
PHST- 2007/08/06 00:00 [received]
PHST- 2007/12/05 00:00 [revised]
PHST- 2007/12/19 00:00 [accepted]
PHST- 2008/04/09 09:00 [pubmed]
PHST- 2008/07/29 09:00 [medline]
PHST- 2008/04/09 09:00 [entrez]
PHST- 2007/12/28 00:00 [pmc-release]
AID - S1286-4579(07)00391-7 [pii]
AID - 10.1016/j.micinf.2007.12.009 [doi]
PST - ppublish
SO  - Microbes Infect. 2008 Apr;10(4):367-73. doi: 10.1016/j.micinf.2007.12.009. Epub 
      2007 Dec 28.